[Detecting immunoreactive GH mRNA in human peripheral blood lymphocytes].

The present study demonstrats that immunoreactive GH mRNA is expressed at low level in normal human peripheral blood lymphocytes by RT-PCR amplification and hybridization. Northern blot analysis of total RNA from lymphocytes with a pituitary hGH cDNA probe revealed a faint transcript migrating almost coincident with pituitary hGH menssenger RNA. Reverse transcription-polymerase chain reaction from total RNA of lymphocytes by using hGH specific primers generated a predicted 702 base pair band with almost the same size as pituitary and also hybridized with pituitary hGH cDNA probe specifically in Southern blot analysis, which indicates a low level expression of GH-like mRNA in human peripheral blood lymphocytes.