Freiman R N, Herr W
Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724, USA.
Genes Dev. 1997 Dec 1;11(23):3122-7. doi: 10.1101/gad.11.23.3122.
Upon infection of human cells, the herpes simplex virus protein VP16 associates with the endogenous cell-proliferation factor HCF. VP16 can also associate with HCFs from invertebrates, suggesting that VP16 mimics a cellular protein whose interaction with HCF has been conserved. Here, we show that VP16 mimics the human basic leucine-zipper protein LZIP, which, through association with HCF, may control cell-cycle progression. VP16 and LZIP share a tetrapeptide motif-D/EHXY-used to associate with human HCF. The LZIP-related Drosophila protein BBF-2/dCREB-A contains this HCF-binding motif, indicating that the LZIP-HCF interaction has been conserved during metazoan evolution.
在人类细胞被感染时，单纯疱疹病毒蛋白VP16会与内源性细胞增殖因子HCF结合。VP16也能与来自无脊椎动物的HCF结合，这表明VP16模拟了一种与HCF相互作用保守的细胞蛋白。在此，我们表明VP16模拟了人类碱性亮氨酸拉链蛋白LZIP，LZIP通过与HCF结合可能控制细胞周期进程。VP16和LZIP共享一个用于与人类HCF结合的四肽基序-D/EHXY。与LZIP相关的果蝇蛋白BBF-2/dCREB-A含有这种HCF结合基序，表明LZIP-HCF相互作用在后生动物进化过程中是保守的。
