Léonard A, Decat G
Can J Genet Cytol. 1976 Mar;18(1):1-10. doi: 10.1139/g76-001.
Densitometric analysis has been performed on mouse chromosomes from "in vivo" preparations stained with Giemsa after trypsinisation. The characteristic curves obtained by analysing the same chromosome after different durations of trypsinisation suggest that a trypsinisation step is not always necessary to get an analysable banding pattern. The best results are obtained when the chromatids are long and closely appressed and by analysing the positive photomicrographs.
已对经胰蛋白酶处理后用吉姆萨染色的“体内”制备的小鼠染色体进行了密度测定分析。对经不同胰蛋白酶处理时间后的同一染色体进行分析所获得的特征曲线表明，并非总是需要胰蛋白酶处理步骤才能获得可分析的带型。当染色单体较长且紧密贴靠，并通过分析正片显微照片时，可获得最佳结果。
