Keherly M J, Papaconstantinou J, McCombs J L
Department of Human Biological Chemistry and Genetics, University of Texas, Medical Branch, Galveston 77555-0359.
Exp Cell Res. 1993 Apr;205(2):361-4. doi: 10.1006/excr.1993.1098.
We describe a sequential staining technique for the karyotypic analysis of interspecific mouse x human somatic cell hybrids. Fluorescence in situ hybridization of samples, previously stained using standard trypsin/Giemsa protocols, was instrumental in the identification of human chromosomes present in hybrid lines. This procedure not only provided a simple distinction between human and mouse chromosomes, but it also allowed the visualization and monitoring of human sequences present in interspecific translocations and subchromosomal fragments.
我们描述了一种用于种间小鼠×人类体细胞杂种核型分析的连续染色技术。对先前使用标准胰蛋白酶/吉姆萨协议染色的样本进行荧光原位杂交，有助于鉴定杂种细胞系中存在的人类染色体。该方法不仅能简单区分人类和小鼠染色体，还能观察和监测种间易位和亚染色体片段中存在的人类序列。
