p53蛋白和流式细胞术DNA分析在浆液性积液研究中的诊断价值。

Diagnostic value of p53 protein and flow cytometric DNA analysis in the study of serous effusions.

作者信息

Lee J S, Lee M C, Park C S, Juhng S W

机构信息

Department of Pathology, Chonnam University Medical School, Kwangju, Seoul, Korea.

出版信息

Acta Cytol. 1997 Nov-Dec;41(6):1719-25. doi: 10.1159/000333175.

DOI:10.1159/000333175

PMID:9390131

Abstract

OBJECTIVE

To investigate the diagnostic value of p53 protein and DNA analysis in the study of serous effusions.

STUDY DESIGN

A total of 76 samples of serous effusions were studied by immunohistochemistry for p53 protein and flow cytometric (FCM) DNA analysis. The results were correlated with final cytologic diagnoses, which were confirmed by immunohistochemistry using antibodies against cytokeratin, carcinoembryonic antigen, epithelial membrane antigen and fibronectin.

RESULTS

Final cytologic diagnoses included 28 malignant effusions and 48 benign effusions. No expression of p53 protein was seen in benign effusions. In contrast, p53 protein expression was seen in 19/28 (sensitivity 68%) malignant effusions. FCM detected aneuploid cells in 12/28 (43% sensitivity) of malignant and 0/46 of benign effusions. Immunohistochemical determination of p53 protein combined with FCM DNA analysis increased sensitivity to 79%.

CONCLUSION

Immunohistochemical determination of p53 protein and FCM DNA analysis can aid in making an accurate and specific diagnosis of serous effusions, but the principal limitation of these tests is their relatively low sensitivity.

摘要

目的

探讨p53蛋白和DNA分析在浆液性积液研究中的诊断价值。

研究设计

采用免疫组织化学法检测76例浆液性积液样本中的p53蛋白，并进行流式细胞术（FCM）DNA分析。将结果与最终细胞学诊断结果进行相关性分析，最终细胞学诊断结果通过使用抗细胞角蛋白、癌胚抗原、上皮膜抗原和纤连蛋白的抗体进行免疫组织化学确认。

结果

最终细胞学诊断包括28例恶性积液和48例良性积液。良性积液中未见p53蛋白表达。相比之下，19/28（敏感性68%）的恶性积液中可见p53蛋白表达。FCM在12/28（敏感性43%）的恶性积液中检测到非整倍体细胞，在46例良性积液中未检测到。免疫组织化学检测p53蛋白结合FCM DNA分析可将敏感性提高到79%。