Risberg B, Davidson B, Nielsen S, Dong H P, Christensen J, Johansen P, Asschenfeldt P, Berner A
Division of Cytology, Department of Pathology, Norwegian Radium Hospital, Oslo, Norway.
Diagn Cytopathol. 2001 Oct;25(4):214-9. doi: 10.1002/dc.2041.
The objective of the present study was to compare the efficiency of immunophenotyping using flow cytometry (FCM) and immunocytochemistry (ICC) in the detection of macrophages in serous effusions. Cytoblock sections from 90 effusions were stained for the monocyte/macrophage marker CD14, using ICC. Fresh-frozen samples of all cases were analyzed for CD14 expression, using FCM. Epithelial, lymphoid, and mesothelial cell populations were identified using antibodies against Ber-EP4, CD45, and N-cadherin, respectively. Results were compared with clinical parameters and morphological diagnosis. Thirty-nine specimens were cytologically diagnosed as malignant, containing tumor cells of nonhematologic origin, whereas 46 were interpreted as benign. Two additional specimens were diagnosed as indeterminate or suspicious for malignancy, and 3 specimens contained lymphoma cells. CD14-positive cells were detected in 85/90 (94%) of effusions using FCM, and in all 90 specimens using ICC. The percentage of CD14-positive cells was highly variable, but in some specimens was as high as 76% using FCM and 85% using ICC. A good association was observed between the two methods in the detection of CD14-positive cells (P < 0.001). The presence of macrophages in effusions showed an association with female gender, using both FCM (P = 0.002) and ICC (P = 0.011), but none with effusion site, patient age, clinical and cytological diagnosis, or presence of Ber-EP4-positive cells (P > 0.05). The presence of Ber-EP4-positive cells showed a strong association with the cytological diagnosis of malignancy (P < 0.001). In conclusion, macrophages are a significant cell population in effusions, of both benign and malignant etiology, due to both their size and their possible confusion with cancer cells. Both FCM and ICC aid in the recognition of these cells, and thus provide an effective tool for the identification of different cell populations in effusions.
本研究的目的是比较流式细胞术（FCM）和免疫细胞化学（ICC）在检测浆液性积液中巨噬细胞时的免疫表型分析效率。对90份积液的细胞块切片进行ICC染色，以检测单核细胞/巨噬细胞标志物CD14。对所有病例的新鲜冷冻样本进行FCM分析，以检测CD14表达。分别使用抗Ber-EP4、CD45和N-钙黏蛋白的抗体来识别上皮细胞、淋巴细胞和间皮细胞群体。将结果与临床参数和形态学诊断进行比较。39份标本经细胞学诊断为恶性，含有非血液学来源的肿瘤细胞，而46份被解释为良性。另外2份标本被诊断为恶性不确定或可疑，3份标本含有淋巴瘤细胞。使用FCM在85/90（94%）的积液中检测到CD14阳性细胞，使用ICC在所有90份标本中均检测到CD14阳性细胞。CD14阳性细胞的百分比变化很大，但在一些标本中，使用FCM时高达76%，使用ICC时高达85%。在检测CD14阳性细胞方面，两种方法之间存在良好的相关性（P < ［］）。使用FCM（P = ［］）和ICC（P = ［］）时，积液中巨噬细胞的存在均与女性性别相关，但与积液部位、患者年龄、临床和细胞学诊断或Ber-EP4阳性细胞的存在均无相关性（P > ［］）。Ber-EP4阳性细胞的存在与恶性肿瘤的细胞学诊断密切相关（P < ［］）。总之，由于巨噬细胞的大小以及它们可能与癌细胞混淆，巨噬细胞是良性和恶性病因所致积液中的重要细胞群体。FCM和ICC都有助于识别这些细胞，从而为识别积液中的不同细胞群体提供了一种有效的工具。 （注：原文中部分P值未给出具体数字）
