Sadhra S, Gray C N, Foulds I S
Institute of Occupational Health, University of Birmingham, Edgbaston, UK.
J Chromatogr B Biomed Sci Appl. 1997 Oct 24;700(1-2):101-10. doi: 10.1016/s0378-4347(97)00293-4.
Rosin is a well recognised skin sensitiser and is also amongst the most common causes of occupational asthma. Due to its complex chemical composition, it is difficult to isolate its many components and this has hindered progress in the identification of the specific respiratory and contact allergens it contains. This paper reports the application of high-performance liquid chromatography and other analytical techniques to the isolation and identification of contact allergens in complex mixtures such as rosin. HPLC methods were developed in order to isolate as many rosin components as possible and these were then patch tested on rosin sensitive individuals. The structure of the most dermatologically active component was then determined using mass spectrometry, nuclear magnetic resonance and infrared techniques. An HPLC method has also been developed which will enable the identification of rosin in commercial products, providing a valuable tool for determining the cause of rosin contact allergy. Furthermore, mass spectral data for the common abieitic-type resin acids are compiled which were used to confirm the identification of the HPLC resin acid peaks and have not been reported previously.
松香是一种公认的皮肤致敏剂，也是职业性哮喘最常见的病因之一。由于其化学成分复杂，很难分离出其众多成分，这阻碍了对其所含特定呼吸道和接触性过敏原的鉴定进展。本文报道了高效液相色谱法和其他分析技术在分离和鉴定松香等复杂混合物中的接触性过敏原方面的应用。开发了高效液相色谱法以尽可能多地分离松香成分，然后将这些成分在对松香敏感的个体上进行斑贴试验。然后使用质谱、核磁共振和红外技术确定最具皮肤活性成分的结构。还开发了一种高效液相色谱法，该方法能够鉴定商业产品中的松香，为确定松香接触过敏的原因提供了有价值的工具。此外，汇编了常见枞酸型树脂酸的质谱数据，用于确认高效液相色谱树脂酸峰的鉴定，这些数据以前未曾报道过。
