Haraguchi Y, Takeuchi Y, Hoshino H
Department of Hygiene and Virology, Gunma University School of Medicine, Maebashi, Japan.
AIDS Res Hum Retroviruses. 1997 Nov 20;13(17):1517-23. doi: 10.1089/aid.1997.13.1517.
We examined the effects of polycations, namely, diethylaminoethyl-dextran (DEAE-dextran) and hexadimethrine bromide (Polybrene), on infection with the retroviruses human T cell leukemia virus types I and II (HTLV-I and HTLV-II) and human immunodeficiency virus type 1 (HIV-1). The plating of vesicular stomatitis virus (VSV) pseudotype bearing envelope antigens of HTLV-I [VSV(HTLV-I)] was inhibited about 2- and 10-fold by treatment with DEAE-dextran and Polybrene, respectively. The formation of HTLV-I viral DNA detected 1 day after infection was also inhibited by these polycations. In contrast, polycations enhanced the plating of the VSV (HTLV-II) pseudotype two- to threefold. The polycations did not affect the plating efficiency of HTLV-I or HTLV-II when added after virus adsorption. Infection of human T cell lines, peripheral blood lymphocytes (PBLs), or brain-derived cells with syncytium-inducing (SI) types of HIV-1 strains (GUN1 and IIIB) was inhibited 3- to 20-fold by polycations. However, infection of PBLs or monocyte-derived macrophages with the macrophage-tropic Ba-L or SF162 strain was enhanced 1.5- to twofold by polycations. On the other hand, syncytium formation in coculture induced by HTLV-I, HTLV-II, or HIV-1 was enhanced two- to threefold unanimously by DEAE-dextran or Polybrene. Although polycations have been used to potentiate human retrovirus adsorption, they inhibited infection of cell-free HTLV-I or SI-type HIV-1 strains.
我们研究了聚阳离子，即二乙氨基乙基葡聚糖（DEAE - 葡聚糖）和溴化己二甲铵（聚凝胺）对逆转录病毒人类T细胞白血病病毒I型和II型（HTLV - I和HTLV - II）以及人类免疫缺陷病毒1型（HIV - 1）感染的影响。携带HTLV - I包膜抗原的水泡性口炎病毒（VSV）假型[VSV(HTLV - I)]的铺板分别用DEAE - 葡聚糖和聚凝胺处理后受到约2倍和10倍的抑制。感染后1天检测到的HTLV - I病毒DNA的形成也受到这些聚阳离子的抑制。相比之下，聚阳离子使VSV(HTLV - II)假型的铺板增加了2至3倍。当在病毒吸附后添加时，聚阳离子不影响HTLV - I或HTLV - II的铺板效率。聚阳离子使人类T细胞系、外周血淋巴细胞（PBLs）或脑源性细胞被合胞体诱导（SI）型HIV - 1毒株（GUN1和IIIB）感染的能力受到3至20倍的抑制。然而，聚阳离子使PBLs或单核细胞衍生的巨噬细胞被嗜巨噬细胞性Ba - L或SF162毒株感染的能力增强了1.5至2倍。另一方面，DEAE - 葡聚糖或聚凝胺一致地使由HTLV - I、HTLV - II或HIV - 1诱导的共培养中的合胞体形成增加了2至3倍。尽管聚阳离子已被用于增强人类逆转录病毒的吸附，但它们抑制了无细胞HTLV - I或SI型HIV - 1毒株的感染。
