Jain N C
Clin Toxicol. 1976;9(2):221-33. doi: 10.3109/15563657608988125.
A rapid, sensitive and specific procedure is described for the mass screening and confirmation of barbiturates in urine specimens. The method is sensitive to 0.5 mug/ml levels of the commonly used barbiturates. The urine samples are screened by radioimmuno-assay. Specimens screened positive are confirmed by gas-liquid chromatography using a flame ionization detector. The barbiturates are analyzed as free acids on a 10% UC W-98 column and as their dimethyl derivatives on a 3% OV-17 column. This dual approach eliminates false positives caused by interfering substances which may be present in the urine, thus resulting in a method specific for the barbiturates.
本文描述了一种用于尿液标本中巴比妥类药物大规模筛查和确证的快速、灵敏且特异的方法。该方法对常用巴比妥类药物的检测灵敏度为0.5微克/毫升。尿液样本通过放射免疫分析法进行筛查。筛查呈阳性的标本采用火焰离子化检测器通过气液色谱法进行确证。巴比妥类药物在10% UC W - 98柱上作为游离酸进行分析，在3% OV - 17柱上作为其二甲基衍生物进行分析。这种双重方法消除了尿液中可能存在的干扰物质导致的假阳性，从而得到一种针对巴比妥类药物的特异方法。
