Jain N C, Sneath T C, Leung W J, Budd R D
J Pharm Sci. 1977 Jan;66(1):66-9. doi: 10.1002/jps.2600660116.
A rapid, sensitive, and specific procedure is described for the mass screening and confirmation of codeine and morphine in urine specimens. The method is sensitive to 0.5-mug/ml levels of both opiates in free and/or conjugate forms. The raw urine is screened directly by radiommunoassay, which is reactive to both free and glucuronide forms of codeine and morphine. Specimens that are screened positive are confirmed by GLC using a flame-ionization detector. The opiates are analyzed as their acetyl derivat-ves on two different columns, OV-25 and Poly-A 103. This multiple approach eliminates false positives caused by interfering substances or structurally similar compounds present in the urine.
本文描述了一种用于尿液样本中可待因和吗啡大规模筛查及确证的快速、灵敏且特异的方法。该方法对游离和/或结合形式的两种阿片类药物的检测灵敏度均为0.5微克/毫升。原始尿液通过放射免疫分析法直接进行筛查，该方法对可待因和吗啡的游离及葡萄糖醛酸结合形式均有反应。筛查呈阳性的样本通过使用火焰离子化检测器的气相色谱法进行确证。阿片类药物以其乙酰衍生物的形式在两根不同的色谱柱（OV - 25和Poly - A 103）上进行分析。这种多重方法消除了由尿液中干扰物质或结构相似化合物导致的假阳性。
