Wynne B, Robertson D
Department of Physiology, University of Western Australia, Nedlands, Australia.
Audiol Neurootol. 1996 Jan-Feb;1(1):54-64.
The noradrenergic (NA) innervation of the superior olivary complex (SOC) was investigated using a monoclonal antibody against dopamine-beta-hydroxylase (D beta H), the synthesizing enzyme for noradrenaline. In addition, positive labelling of D beta H in the inferior colliculus, the lateral lemniscus (LL) and the cochlear nucleus were documented. The antibody was detected using the highly specific and sensitive ABC immunocytochemical method. The specific aim of the study was to show the distribution of putative NA cells and fibres in the SOC in greater detail than had been previously reported. All investigated auditory regions contained D beta H-positive fibres in varying densities. All superior olivary and periolivary nuclei showed NA innervation by mainly fine, weakly staining fibres. Both nuclei of the LL were also found to contain D beta H-positive fibres. D beta H-positive cell bodies were found in the region of the ventral lateral lemniscus and immediately lateral and dorsal to the lateral superior olivary nucleus. Thus the NA innervation of the superior olivary complex could originate from these regions and/or the locus coeruleus.
利用抗多巴胺β-羟化酶（DβH）的单克隆抗体对橄榄上复合体（SOC）的去甲肾上腺素能（NA）神经支配进行了研究，多巴胺β-羟化酶是去甲肾上腺素的合成酶。此外，还记录了下丘、外侧丘系（LL）和耳蜗核中DβH的阳性标记。使用高度特异性和敏感性的ABC免疫细胞化学方法检测该抗体。该研究的具体目的是比以往报道更详细地显示假定的NA细胞和纤维在SOC中的分布。所有研究的听觉区域都含有不同密度的DβH阳性纤维。所有橄榄上核和橄榄周核均显示有NA神经支配，主要由细小、弱染色的纤维组成。还发现LL的两个核都含有DβH阳性纤维。在腹侧外侧丘系区域以及外侧上橄榄核的外侧和背侧紧邻处发现了DβH阳性细胞体。因此，橄榄上复合体的NA神经支配可能起源于这些区域和/或蓝斑。
