Wang J S, Jen C J, Chen H I
Department of Physiology, National Cheng-Kung University Medical College, Tainan, Taiwan 701, Republic of China.
J Appl Physiol (1985). 1997 Dec;83(6):2080-5. doi: 10.1152/jappl.1997.83.6.2080.
To investigate the effects of chronic exercise and deconditioning on platelet function in women, 16 healthy sedentary women were divided into control and exercise groups. The exercise group cycled on an ergometer at 50% maximal oxygen consumption for 30 min/day, 5 days/wk, for two consecutive menstrual cycles and then were deconditioned for three menstrual cycles. During this period, platelet adhesiveness on a fibrinogen-coated surface, ADP-induced platelet aggregation and intracellular calcium concentration elevation, guanosine 3',5'-cyclic monophosphate (cGMP) content in platelets, and plasma nitric oxide metabolite levels were measured before and immediately after a progressive exercise test in the midfollicular phase. Our results indicated that, after exercise training, 1) resting heart rates and blood pressures were reduced, and exercise performance was improved; 2) resting platelet function was decreased, whereas plasma nitrite and nitrate levels and platelet cGMP contents were enhanced; and 3) the potentiation of platelet function by acute strenuous exercise was decreased, whereas the increases in plasma nitrite and nitrate levels and platelet cGMP contents were enhanced by acute exercise. Furthermore, deconditioning reversed these training effects. This implies that training-induced platelet functional changes in women in the midfollicular phase may be mediated by nitric oxide.
为研究长期运动和去适应对女性血小板功能的影响，将16名久坐不动的健康女性分为对照组和运动组。运动组在测力计上以最大耗氧量的50%进行骑行，每天30分钟，每周5天，持续两个连续月经周期，然后进行三个月经周期的去适应。在此期间，在卵泡中期进行递增运动试验之前和之后立即测量血小板在纤维蛋白原包被表面的黏附性、ADP诱导的血小板聚集以及细胞内钙浓度升高、血小板中鸟苷3',5'-环磷酸（cGMP）含量和血浆一氧化氮代谢物水平。我们的结果表明，运动训练后，1）静息心率和血压降低，运动能力提高；2）静息血小板功能降低，而血浆亚硝酸盐和硝酸盐水平以及血小板cGMP含量增加；3）急性剧烈运动对血小板功能的增强作用降低，而急性运动使血浆亚硝酸盐和硝酸盐水平以及血小板cGMP含量增加。此外，去适应逆转了这些训练效果。这意味着卵泡中期女性训练诱导的血小板功能变化可能由一氧化氮介导。
