非糖化和糖化胰高血糖素样肽-1（7-36）酰胺对分离的小鼠腹部肌肉葡萄糖代谢的影响

Effects of non-glycated and glycated glucagon-like peptide-1(7-36) amide on glucose metabolism in isolated mouse abdominal muscle.

作者信息

O'Harte F P, Gray A M, Abdel-Wahab Y H, Flatt P R

机构信息

School of Biomedical Sciences, University of Ulster, Coleraine, N. Ireland.

出版信息

Peptides. 1997;18(9):1327-33. doi: 10.1016/s0196-9781(97)00201-5.

DOI:10.1016/s0196-9781(97)00201-5

PMID:9392833

Abstract

This study investigated the actions of non-glycated and glycated glucagon-like peptide-1(7-36)amide (tGLP-1) on glucose uptake and metabolism in isolated mouse abdominal muscle. Monoglycated tGLP-1 (Mr 3463.8) was prepared under hyperglycemic reducing conditions and purified by HPLC. Non-glycated tGLP-1 (10(-10)-10(-8) mol/l) stimulated both 2-deoxy-D-[1-3H]glucose uptake (1.3-1.5 fold) and 14C-glucose oxidation (1.4-1.7 fold) in muscle compared to controls without tGLP-1. Glycation reduced these stimulatory effects by 27-33% and 25% (at 10(-9) mol/l), respectively. tGLP-1 (10(-10)-10(-8) mol/l) promoted muscle glycogenesis and lactate production, whereas glycated peptide was ineffective below 10(-9) mol/l. This study demonstrates that tGLP-1 has potent glycogenic effects in mouse abdominal muscle in vitro and that glycation impairs its action.

摘要

本研究调查了非糖化和糖化胰高血糖素样肽-1(7-36)酰胺（tGLP-1）对分离的小鼠腹部肌肉葡萄糖摄取和代谢的作用。单糖化tGLP-1（分子量3463.8）在高血糖还原条件下制备，并通过高效液相色谱法纯化。与无tGLP-1的对照组相比，非糖化tGLP-1（10⁻¹⁰ - 10⁻⁸mol/L）刺激肌肉中2-脱氧-D-[1-³H]葡萄糖摄取（1.3 - 1.5倍）和¹⁴C-葡萄糖氧化（1.4 - 1.7倍）。糖化分别使这些刺激作用降低了27 - 33%和25%（在10⁻⁹mol/L时）。tGLP-1（10⁻¹⁰ - 10⁻⁸mol/L）促进肌肉糖原生成和乳酸生成，而糖化肽在低于10⁻⁹mol/L时无效。本研究表明，tGLP-1在体外对小鼠腹部肌肉具有强大的糖原生成作用，且糖化会损害其作用。