Cicconetti F, Teodori L, Persiani M, Di Tondo U, Alò P, Marci A, Brun S, Göhde W
Clinic of Surgery, University of Rome La Sapienza, Italy.
J Cancer Res Clin Oncol. 1997;123(10):571-7. doi: 10.1007/s004320050107.
The present study was performed to improve early lung cancer diagnosis in bronchial washing fluid, thereby increasing the diagnostic sensitivity of bronchoscopy by means of high-resolution flow cytometry (FC). We combined dual-parameter DNA/protein FC and conventional cytology in bronchial washing fluid samples from 112 patients with neoplastic and non-neoplastic lung diseases and found 43% of histologically confirmed tumor cases to be cytologically positive; 63% of the tumor samples were aneuploid, 52% of the aneuploid cases were cytologically positive and 48% were negative. In the negative cases, FC was an independent diagnostic factor. In 32% of the cases, FC also failed to detect abnormalities. However, the combination of both techniques increased the sensitivity in detecting neoplastic cells to 73%. Furthermore, simultaneous DNA/protein analysis allowed the recognition of aneuploid cell lines not detectable by single DNA measurement. Identification of aneuploid subpopulations by dual-parameter analysis in cytologically negative one-parameter FC "diploid" samples assumes an important diagnostic value. Dual-parameter DNA/protein FC is a valuable technique that increases the diagnostic yield of bronchoscopy with no risk for the patient and a low additional cost.
本研究旨在改善支气管灌洗液中早期肺癌的诊断，从而通过高分辨率流式细胞术（FC）提高支气管镜检查的诊断敏感性。我们将双参数DNA/蛋白质FC与传统细胞学方法相结合，对112例患有肿瘤性和非肿瘤性肺部疾病患者的支气管灌洗液样本进行检测，发现43%经组织学确诊的肿瘤病例在细胞学检查中呈阳性；63%的肿瘤样本为非整倍体，52%的非整倍体病例在细胞学检查中呈阳性，48%呈阴性。在阴性病例中，FC是一个独立的诊断因素。在32%的病例中，FC也未能检测到异常情况。然而，两种技术相结合将检测肿瘤细胞的敏感性提高到了73%。此外，同时进行DNA/蛋白质分析能够识别单DNA测量无法检测到的非整倍体细胞系。在细胞学检查为阴性的单参数FC“二倍体”样本中，通过双参数分析鉴定非整倍体亚群具有重要的诊断价值。双参数DNA/蛋白质FC是一项有价值的技术，它提高了支气管镜检查的诊断率，对患者无风险且额外成本较低。
