由异鸟嘌呤四联体形成的四链DNA：复杂化学计量、热稳定性及对外切核酸酶的抗性

Four-stranded DNA formed by isoguanine quartets: complex stoichiometry, thermal stability and resistance against exonucleases.

作者信息

Seela F, Wei C, Melenewski A

机构信息

Laboratorium für Organische und Bioorganische Chemie, Universität Osnabrück, Germany.

出版信息

Orig Life Evol Biosph. 1997 Dec;27(5-6):597-608. doi: 10.1023/a:1006531728815.

DOI:10.1023/a:1006531728815

PMID:9394471

Abstract

Single stranded DNA-fragments containing short runs of isoguanine such as d(T4iG4T4) (5) or d(iG4T4) (6) form quartet structures by self-assembly of the isoguanine residues. The stoichiometry of the complexes is deduced from mixed aggregates formed between d(T4iG4T4) and d(iG4T4). The iGd-tetrads are more stable with regard to their thermal denaturation and to their resistance against enzymatic phosphodiester hydrolysis than those formed by dG.

摘要

含有短链异鸟嘌呤（如d(T4iG4T4) (5) 或d(iG4T4) (6)）的单链DNA片段通过异鸟嘌呤残基的自组装形成四重结构。复合物的化学计量是从d(T4iG4T4)和d(iG4T4)之间形成的混合聚集体推导出来的。与由dG形成的四重体相比，iGd-四联体在热变性及其对酶促磷酸二酯水解的抗性方面更稳定。