Galassi L, Pica G
Basic Appl Histochem. 1979;23(2):149-53.
Autoradiographs were prepared of sections of the ovary of Dytiscus marginalis labelled with thymidine-3H after each successive step of the Feulgen reaction and after treatment with each separate component of the Schiff's reagent. Results of grain counts over ovarian nurse cells showed that losses of thymidine-3H activity occur not only during hydrolysis but also during the successive steps of the Feulgen reaction. It is suggested that the latter decrease in radioactivity may depend on the extraction of fragments of apurinic acid from the sections. An emulsion desensitizing effect has also been observed in sections stained with basic fuchsin alone; this effect appears, however, to be strongly counteracted by the metabisulphite present in the Schiff's reagent.
在福尔根反应的每一个连续步骤之后，以及在用席夫试剂的每一种单独成分处理之后，制备了用氚化胸腺嘧啶核苷标记的黄缘龙虱卵巢切片的放射自显影片。对卵巢滋养细胞的颗粒计数结果表明，氚化胸腺嘧啶核苷活性的损失不仅发生在水解过程中，而且也发生在福尔根反应的连续步骤中。有人提出，放射性的后一种降低可能取决于从切片中提取无嘌呤酸片段。在用碱性品红单独染色的切片中也观察到了乳剂脱敏效应；然而，席夫试剂中存在的焦亚硫酸盐似乎强烈抵消了这种效应。
