Kobayashi K, Morioka Y, Isaka Y, Tozawa T
Diagnostic Science Division, Shionogi & Co., Ltd., Settsu.
Rinsho Byori. 1997 Nov;45(11):1062-6.
Uric acid in blood has been widely accepted as a reliable indicator of hyperuricemia and gout, and its assay method has been established. In the present study, we developed a simple and non-invasive rapid method for the determination of uric acid in hair. Concentration(nmol/mg hair) of uric acid extracted from 10-20 mg of hair(95% < extractability; 0.1N KOH, 37 degrees C, 2 hr) was determined by an enzymatic method using uricase. The concentration of uric acid(nmol/mg hair, mean +/- SD: 0.489 +/- 0.157, n = 16) in hair from hyperuricemic patients was significantly higher than that(0.258 +/- 0.107, n = 8) from healthy volunteers(p < 0.01). Within-run and between-day precisions(reproducibilities, CVs) for the assay were 9.6-10.3%(n = 10 each) and 11.6-16.3%(n = 7 each), respectively. The concentration(y, nmol/mg hair) of uric acid in hair correlated well with that in blood(x, g/l): y = 8.770x-0.123(r = 0.746, Syx = 0.122, n = 23). Changes in the concentration of uric acid in hair of hyperuricemic patient treated with allopurinol paralleled to those in blood. In conclusion, it was confirmed that the concentration of uric acid in hair reflected that in blood, suggesting that measuring uric acid in hair can be available for the metabolic control in hyperuricemia.
血液中的尿酸已被广泛公认为是高尿酸血症和痛风的可靠指标，其检测方法也已确立。在本研究中，我们开发了一种简单、无创的快速方法来测定头发中的尿酸。从10 - 20毫克头发中提取的尿酸（95% < 提取率；0.1N氢氧化钾，37摄氏度，2小时），通过使用尿酸酶的酶法测定其浓度（纳摩尔/毫克头发）。高尿酸血症患者头发中的尿酸浓度（纳摩尔/毫克头发，均值±标准差：0.489 ± 0.157，n = 16）显著高于健康志愿者（0.258 ± 0.107，n = 8）（p < 0.01）。该检测方法的批内和批间精密度（重现性，变异系数）分别为9.6 - 10.3%（每组n = 10）和11.6 - 16.3%（每组n = 7）。头发中尿酸的浓度（y，纳摩尔/毫克头发）与血液中尿酸的浓度（x，克/升）相关性良好：y = 8.770x - 0.123（r = 0.746，Syx = 0.122，n = 23）。用别嘌呤醇治疗的高尿酸血症患者头发中尿酸浓度的变化与血液中的变化平行。总之，已证实头发中的尿酸浓度反映了血液中的尿酸浓度，这表明检测头发中的尿酸可用于高尿酸血症的代谢控制。
