Henkart M, Landis D M, Reese T S
J Cell Biol. 1976 Aug;70(2 pt 1):338-47. doi: 10.1083/jcb.70.2.338.
The structure of membranes at junctions between the plasma membrane and underlying cisterns of endoplasmic reticulum in amphioxus muscle and mouse cerebellar neurons was studied using the freeze-fracture technique. In amphioxus muscle, subsurface cisterns of sarcoplasmic reticulum form junctions with the surface membrane at the level of the sarcomere I bands. On the protoplasmic leaflet of the sarcolemma overlying these junctions were aggregates of large particles. On the protoplasmic leaflet of the membranes of cerebellar basket, stellate and Purkinie cells there were similar aggregates of large particles. In both tissues, the corresponding external membrane halves had arrays of pits apparently complementary to the aggregates of large particles. Cross fractures through junctions showed that the particle aggregates in neuronal and muscle membranes were consistently located over intracellular cisterns closely applied to the plasma membrane. Thus, a similar plasma membrane specialization is found at subsurface cisterns in mammalian neurons and amphioxus muscle. This similarity supports the hypothesis that subsurface cisterns in neurons, like those in muscle, couple some intracellular activity to the electrical activity of the plasma membrane.
运用冷冻蚀刻技术研究了文昌鱼肌肉和小鼠小脑神经元中质膜与内质网池之间连接处的膜结构。在文昌鱼肌肉中，肌浆网的表面下池在肌节I带水平与表面膜形成连接。在覆盖这些连接处的肌膜原生质小叶上有大颗粒聚集体。在小脑篮状细胞、星状细胞和浦肯野细胞的膜原生质小叶上也有类似的大颗粒聚集体。在这两种组织中，相应的外膜半叶有明显与大颗粒聚集体互补的凹坑阵列。通过连接处的交叉断裂显示，神经元和肌肉膜中的颗粒聚集体始终位于紧贴质膜的细胞内池上方。因此，在哺乳动物神经元和文昌鱼肌肉的表面下池中发现了类似的质膜特化。这种相似性支持了这样一种假说，即神经元中的表面下池与肌肉中的表面下池一样，将一些细胞内活动与质膜的电活动联系起来。
