Versland M R, Wu G Y, Gorelick F S, Larkin J M
Department of Medicine, University of Connecticut Health Center, Farmington 06030, USA.
Dig Dis Sci. 1997 Nov;42(11):2246-53. doi: 10.1023/a:1018810500006.
In rats, serum secretory component (SC) is elevated in mechanical but not hepatocellular cholestasis. To determine if serum SC might distinguish cholestatic syndromes in humans, serum samples were obtained from control subjects and patients with mechanical and hepatocellular cholestasis. Equal volumes of serum were assayed for SC by immunoblotting with an antibody specific for human SC. Quantitative densitometry of these immunoblots showed that in mechanically obstructed patients serum SC was reversibly elevated to a level approximately 10-fold higher than that of patients with hepatocellular cholestasis (P < 0.001). When comparing the two cholestatic groups, levels of serum alkaline phosphatase, but not bilirubin and alanine aminotransferase, were significantly higher in the group with mechanical cholestasis (P < 0.01). When comparing individual patients, serum SC was more reliable than alkaline phosphatase in distinguishing the two cholestatic syndromes (P < 0.05). Thus, serum SC may distinguish mechanical from hepatocellular cholestasis in humans.
在大鼠中，机械性胆汁淤积而非肝细胞性胆汁淤积时血清分泌成分（SC）会升高。为了确定血清SC是否可用于区分人类的胆汁淤积综合征，我们采集了对照组以及患有机械性和肝细胞性胆汁淤积患者的血清样本。通过使用针对人SC的特异性抗体进行免疫印迹法，对等量血清中的SC进行检测。这些免疫印迹的定量光密度分析显示，在机械性梗阻患者中，血清SC可逆性升高至比肝细胞性胆汁淤积患者高约10倍的水平（P < 0.001）。比较两个胆汁淤积组时，机械性胆汁淤积组的血清碱性磷酸酶水平显著高于肝细胞性胆汁淤积组，但胆红素和丙氨酸转氨酶水平无显著差异（P < 0.01）。在比较个体患者时，血清SC在区分这两种胆汁淤积综合征方面比碱性磷酸酶更可靠（P < 0.05）。因此，血清SC可能有助于区分人类的机械性胆汁淤积和肝细胞性胆汁淤积。
