Misztal G, Hopkała H
Department of Medicinal Chemistry, Medical Academy, Lublin, Poland.
Pharmazie. 1997 Nov;52(11):854-6.
A rapid, simple, accurate method for the determination of fluoxetine in human plasma is presented. Liquid-liquid extraction of fluoxetine was carried out using diethyl ether. Chlorprothixene was applied as an internal standard. The samples were chromatographed on a LiChrosorb RP-18 (10 microns) column and the mobile phase was acetonitrile/phosphate buffer pH 2.70 (9:1). The detection was carried at 254 nm. A linear quantitative response curve was generated over a concentration range of 100-600 ng/ml. Overall extraction efficiency of the extraction procedure was found to be 86 to 91% with a correlation coefficient of 0.992.
本文介绍了一种快速、简单、准确测定人血浆中氟西汀的方法。采用乙醚进行氟西汀的液-液萃取。以氯普噻吨作为内标。样品在LiChrosorb RP-18（10微米）柱上进行色谱分析，流动相为乙腈/ pH 2.70的磷酸盐缓冲液（9:1）。检测波长为254 nm。在100 - 600 ng/ml的浓度范围内生成线性定量响应曲线。萃取过程的总体萃取效率为86%至91%，相关系数为0.992。
