从芽孢杆菌属KSM-635中分离出的截短型A家族碱性内切葡聚糖酶的结晶及初步X射线分析
Crystallization and preliminary X-ray analysis of a truncated family A alkaline endoglucanase isolated from Bacillus sp. KSM-635.
作者信息
Shirai T, Yamane T, Hidaka T, Kuyama K, Suzuki A, Ashida T, Ozaki K, Ito S
机构信息
Department of Biotechnology and Biomaterial Chemistry, Graduate School of Engineering, Nagoya University.
出版信息
J Biochem. 1997 Oct;122(4):683-5. doi: 10.1093/oxfordjournals.jbchem.a021808.
The catalytic domain of an alkaline endo-1,4-beta-glucanase (family A) isolated from Bacillus sp. KSM-635 (Mr = 40.2 kDa) was crystallized using the hanging drop vapor diffusion method. Two different crystal forms were obtained. Form 2 crystals (trigonal space group R3 with cell dimensions of a = b = 111.9 and c = 207.1 angstroms in a hexagonal lattice) were found to be more stable than form 1 ones upon X-ray irradiation. A full data set for form 2 crystals has been collected up to 3.3 angstroms resolution.
从芽孢杆菌属KSM - 635（分子量 = 40.2 kDa）中分离得到的碱性内切 - 1,4 - β - 葡聚糖酶（A家族）的催化结构域，采用悬滴气相扩散法进行结晶。获得了两种不同的晶体形式。发现2型晶体（三方晶系空间群R3，在六方晶格中晶胞参数a = b = 111.9 Å，c = 207.1 Å）在X射线照射下比1型晶体更稳定。已收集到2型晶体分辨率高达3.3 Å的完整数据集。
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