Paul M, Johnson J D, Epel D
J Exp Zool. 1976 Jul;197(1):127-33. doi: 10.1002/jez.1401970115.
Sea urchin eggs treated with 10 mM NHC1 release a "fertilization acid" although cortical granule exocytosis does not take place. If the eggs are inseminated following ammonia activation, the cortical reaction occurs and a fertilization membrane elevates in the absence of detectable acid release. Examination by electron microscopy of eggs fixed between ammonia activation and insemination confirms the presence of intact granules. Thus, the fertilization acid is not caused by the release of the cortical granules. Ammonia treatment of fertilized eggs stimulates further release of acid. The release of acid can occur repeatedly in ammonia activated eggs if they are washed into normal seawater between successive ammonia treatments. Our results suggest that the release of fertilization acid is related to some metabolic process which can be turned "on or off".
用10 mM氯化铵处理的海胆卵会释放出一种“受精酸”，尽管皮质颗粒胞吐作用并未发生。如果在氨激活后对卵进行授精，皮质反应会发生，并且在没有可检测到的酸释放的情况下受精膜会升高。对在氨激活和授精之间固定的卵进行电子显微镜检查证实存在完整的颗粒。因此，受精酸不是由皮质颗粒的释放引起的。对受精卵进行氨处理会刺激酸的进一步释放。如果在连续的氨处理之间将氨激活的卵冲洗到正常海水中，酸的释放在氨激活的卵中会反复发生。我们的结果表明，受精酸的释放与某种可以被“开启或关闭”的代谢过程有关。
