Czaja A J, Kruger M, Santrach P J, Moore S B, Manns M P
Department of Laboratory Medicine and Pathology, Mayo Clinic and Mayo Foundation, Rochester, Minnesota 55905, USA.
Am J Gastroenterol. 1997 Dec;92(12):2197-200.
Our aim was to determine whether alleles affecting susceptibility to type 1 autoimmune hepatitis in the United States occur as commonly in German patients with type 2 disease.
DNA specimens from 12 German patients with type 2 autoimmune hepatitis were tested for class II alleles of the major histocompatibility complex by polymerase chain reaction using sequence specific primers. Eighty-six American patients with type 1 disease and 102 Caucasoid normal subjects from the United States were tested in a similar manner.
American patients with type 1 autoimmune hepatitis had DRB103 alleles more commonly than the German patients with type 2 disease (51% vs 17%, p = 0.03) and DRB10301 occurred more frequently in the type 1 patients (51% vs 17%, p = 0.03). The frequency of DRB104 alleles was also higher in the type 1 patients after exclusion of the DR103 alleles (64% vs 20%, p = 0.01). In contrast, patients with type 2 disease more commonly had DRB107 (p = 0.003), DRB115 (p = 0.004), and DQB106 (p = 0.0004). DRB107 (p = 0.005), DRB401 (p = 0.03), and DQB106 (p = 0.03) also occurred more frequently in the type 2 patients from Germany than in the normal subjects from the United States, although none of these frequencies were statistically significant by an adjusted p value.
German patients with type 2 autoimmune hepatitis do not have the same susceptibility alleles as American patients with type 1 disease. Regional differences in prevalence may reflect the genetic profiles of the populations at risk.
我们的目的是确定在美国影响1型自身免疫性肝炎易感性的等位基因在德国2型疾病患者中是否同样常见。
采用序列特异性引物聚合酶链反应，对12例德国2型自身免疫性肝炎患者的DNA样本进行主要组织相容性复合体II类等位基因检测。以类似方式检测了86例美国1型疾病患者和102名来自美国的白种正常受试者。
美国1型自身免疫性肝炎患者中DRB103等位基因比德国2型疾病患者更常见（51%对17%，p = 0.03），且DRB10301在1型患者中出现频率更高（51%对17%，p = 0.03）。排除DR103等位基因后，1型患者中DRB104等位基因频率也更高（64%对20%，p = 0.01）。相比之下，2型疾病患者更常见有DRB107（p = 0.003）、DRB115（p = 0.004）和DQB106（p = 0.0004）。DRB107（p = 0.005）、DRB401（p = 0.03）和DQB106（p = 0.03）在德国2型患者中也比在美国正常受试者中出现频率更高，尽管经校正p值后这些频率均无统计学意义。
德国2型自身免疫性肝炎患者与美国1型疾病患者没有相同的易感性等位基因。患病率的区域差异可能反映了高危人群的基因谱。
