在P2样大肠杆菌噬菌体高度可变位点内鉴定出一个编码功能性逆转录酶的基因。
Identification of a gene encoding a functional reverse transcriptase within a highly variable locus in the P2-like coliphages.
作者信息
Odegrip Richard, Nilsson Anders S, Haggård-Ljungquist Elisabeth
机构信息
Department of Genetics, Microbiology and Toxicology, Stockholm University, S-106 91 Stockholm, Sweden.
出版信息
J Bacteriol. 2006 Feb;188(4):1643-7. doi: 10.1128/JB.188.4.1643-1647.2006.
Abstract
The P2-like coliphages are highly similar; the structural genes show at least 96% identity. However, at two loci they have genes believed to be horizontally transferred. We show that the genetic content at the second loci, the TO region, contains six completely different sequences with high AT contents and with different open reading frames. The product of one of them exhibits reverse transcriptase activity and blocks infection of phage T5.
摘要
P2样大肠杆菌噬菌体高度相似；其结构基因显示至少96%的同一性。然而，在两个位点它们有被认为是水平转移的基因。我们表明，第二个位点即TO区域的遗传内容包含六个完全不同的序列，具有高AT含量和不同的开放阅读框。其中一个的产物表现出逆转录酶活性并阻断噬菌体T5的感染。
相似文献
1
Identification of a gene encoding a functional reverse transcriptase within a highly variable locus in the P2-like coliphages.
J Bacteriol. 2006 Feb;188(4):1643-7. doi: 10.1128/JB.188.4.1643-1647.2006.
2
Site-specific recombination links the evolution of P2-like coliphages and pathogenic enterobacteria.
Mol Biol Evol. 2004 Jan;21(1):1-13. doi: 10.1093/molbev/msg223. Epub 2003 Aug 29.
3
Genomic analysis and relatedness of P2-like phages of the Burkholderia cepacia complex.
BMC Genomics. 2010 Oct 25;11:599. doi: 10.1186/1471-2164-11-599.
4
Functions involved in bacteriophage P2-induced host cell lysis and identification of a new tail gene.
J Bacteriol. 1994 Aug;176(16):4974-84. doi: 10.1128/jb.176.16.4974-4984.1994.
5
The late-expressed region of the temperate coliphage 186 genome.
Virology. 1998 Aug 15;248(1):117-30. doi: 10.1006/viro.1998.9263.
6
The complete nucleotide sequence of bacteriophage HP1 DNA.
Nucleic Acids Res. 1996 Jun 15;24(12):2360-8. doi: 10.1093/nar/24.12.2360.
7
Molecular cloning and characterization of bacteriophage P2 genes R and S involved in tail completion.
Virology. 1994 May 1;200(2):347-59. doi: 10.1006/viro.1994.1199.
8
Retronphage phi R73: an E. coli phage that contains a retroelement and integrates into a tRNA gene.
Science. 1991 May 17;252(5008):969-71. doi: 10.1126/science.1709758.
9
Derepression of prophage P2 by satellite phage P4: cloning of the P4 epsilon gene and identification of its product.
J Virol. 1997 Jun;71(6):4502-8. doi: 10.1128/JVI.71.6.4502-4508.1997.
10
Cloning and characterization of bacteriophage-like DNA from Haemophilus somnus homologous to phages P2 and HP1.
J Bacteriol. 1997 Mar;179(6):1872-9. doi: 10.1128/jb.179.6.1872-1879.1997.
引用本文的文献
1
Structure-functional characterization of Lactococcus AbiA phage defense system.
Nucleic Acids Res. 2024 May 8;52(8):4723-4738. doi: 10.1093/nar/gkae230.
2
A functional selection reveals previously undetected anti-phage defence systems in the E. coli pangenome.
Nat Microbiol. 2022 Oct;7(10):1568-1579. doi: 10.1038/s41564-022-01219-4. Epub 2022 Sep 19.
3
Mechanism of protein-primed template-independent DNA synthesis by Abi polymerases.
Nucleic Acids Res. 2022 Sep 23;50(17):10026-10040. doi: 10.1093/nar/gkac772.
4
UG/Abi: a highly diverse family of prokaryotic reverse transcriptases associated with defense functions.
Nucleic Acids Res. 2022 Jun 24;50(11):6084-6101. doi: 10.1093/nar/gkac467.
5
Recruitment of Mobile Genetic Elements for Diverse Cellular Functions in Prokaryotes.
Front Mol Biosci. 2022 Mar 24;9:821197. doi: 10.3389/fmolb.2022.821197. eCollection 2022.
6
Phages and their satellites encode hotspots of antiviral systems.
Cell Host Microbe. 2022 May 11;30(5):740-753.e5. doi: 10.1016/j.chom.2022.02.018. Epub 2022 Mar 21.
7
Identification and classification of reverse transcriptases in bacterial genomes and metagenomes.
Nucleic Acids Res. 2022 Mar 21;50(5):e29. doi: 10.1093/nar/gkab1207.
8
Prokaryotic reverse transcriptases: from retroelements to specialized defense systems.
FEMS Microbiol Rev. 2021 Nov 23;45(6). doi: 10.1093/femsre/fuab025.
9
Expanding Diversity of Single-Strand Annealing Proteins: A Putative Role of Bacteriophage-Host Arms Race.
Front Microbiol. 2021 Apr 20;12:644622. doi: 10.3389/fmicb.2021.644622. eCollection 2021.
10
Systematic prediction of genes functionally associated with bacterial retrons and classification of the encoded tripartite systems.
Nucleic Acids Res. 2020 Dec 16;48(22):12632-12647. doi: 10.1093/nar/gkaa1149.
本文引用的文献
1
Expression and site-directed mutagenesis of the lactococcal abortive phage infection protein AbiK.
J Bacteriol. 2005 Jun;187(11):3721-30. doi: 10.1128/JB.187.11.3721-3730.2005.
2
Lactococcal phage genes involved in sensitivity to AbiK and their relation to single-strand annealing proteins.
J Bacteriol. 2004 Jun;186(11):3649-52. doi: 10.1128/JB.186.11.3649-3652.2004.
3
Lysogeny.
Adv Virus Res. 1958;5:151-93. doi: 10.1016/s0065-3527(08)60673-9.
4
Suppression of the multiplication of heterologous bacteriophages in lysogenic bacteria.
Virology. 1957 Jun;3(3):496-513. doi: 10.1016/0042-6822(57)90006-5.
5
Site-specific recombination links the evolution of P2-like coliphages and pathogenic enterobacteria.
Mol Biol Evol. 2004 Jan;21(1):1-13. doi: 10.1093/molbev/msg223. Epub 2003 Aug 29.
6
Evidence for horizontal transfer of the EcoT38I restriction-modification gene to chromosomal DNA by the P2 phage and diversity of defective P2 prophages in Escherichia coli TH38 strains.
J Bacteriol. 2003 Apr;185(7):2296-305. doi: 10.1128/JB.185.7.2296-2305.2003.
7
Complete genome sequence of Salmonella enterica serovar Typhimurium LT2.
Nature. 2001 Oct 25;413(6858):852-6. doi: 10.1038/35101614.
8
Characterization of the major control region of Vibrio cholerae bacteriophage K139: immunity, exclusion, and integration.
J Bacteriol. 1999 May;181(9):2902-13. doi: 10.1128/JB.181.9.2902-2913.1999.
9
The complete nucleotide sequence of phi CTX, a cytotoxin-converting phage of Pseudomonas aeruginosa: implications for phage evolution and horizontal gene transfer via bacteriophages.
Mol Microbiol. 1999 Jan;31(2):399-419. doi: 10.1046/j.1365-2958.1999.01158.x.
10
msDNA-Ec48, the smallest multicopy single-stranded DNA from Escherichia coli.
J Bacteriol. 1997 Dec;179(24):7865-8. doi: 10.1128/jb.179.24.7865-7868.1997.
Zotero 插件