Hannigan J H, Hackett J A, Tilak J, Subramanian M G
Department of Obstetrics & Gynecology, C. S. Mott Center for Human Growth and Development, Wayne State University School of Medicine, Detroit, MI 48201, USA.
Alcohol. 1997 Nov-Dec;14(6):585-92. doi: 10.1016/s0741-8329(97)00053-0.
We examined the impact of prenatal alcohol exposure on serum prolactin levels and on the ability of the D2 dopamine antagonist sulpiride to stimulate prolactin release in Long-Evans rats. Pregnant rats were intubated with alcohol (0, 3, or 5 g/kg/day) from gestational day 8 (GD8) to GD20. Adult female offspring were screened for estrous cycle stage. At diestrus, the rats were challenged with a single dose of sulpiride (0, 10, or 40 micrograms/kg) and trunk blood was collected 20 min later. After prenatal exposure to either dose of alcohol, mean basal serum levels of prolactin were about 65% less than the 0 g/kg group, and the 35-40% mean differences from an untreated control group were not significant. Sulpiride produced dramatic dose-dependent increases in serum prolactin levels in all prenatal treatment groups. Across all doses of sulpiride, the group given the higher dose of prenatal alcohol (5 g/kg/day) had significantly lower serum prolactin levels than all other groups. There was no significant interaction between prenatal treatment and sulpiride dose. Neither prenatal alcohol exposure nor sulpiride injections had significant effects on serum corticosterone levels in this study. Although the current results are unclear regarding a baseline decrease in prolactin levels after prenatal alcohol exposure, the overall results suggest that prenatal alcohol exposure decreases prolactin levels but there is no evidence that it does so by altering dopaminergic tone in hypothalamus of female rats.
我们研究了产前酒精暴露对长 Evans 大鼠血清催乳素水平以及 D2 多巴胺拮抗剂舒必利刺激催乳素释放能力的影响。妊娠大鼠从妊娠第 8 天（GD8）至 GD20 经插管给予酒精（0、3 或 5 g/kg/天）。对成年雌性后代进行动情周期阶段筛查。在动情间期，给大鼠单次注射舒必利（0、10 或 40 微克/千克），20 分钟后采集躯干血。产前暴露于任一剂量酒精后，催乳素的平均基础血清水平比 0 g/kg 组低约 65%，与未处理对照组的平均差异 35 - 40%无统计学意义。在所有产前治疗组中，舒必利均使血清催乳素水平产生显著的剂量依赖性升高。在所有舒必利剂量组中，产前给予较高剂量酒精（5 g/kg/天）的组血清催乳素水平显著低于所有其他组。产前治疗与舒必利剂量之间无显著交互作用。在本研究中，产前酒精暴露和舒必利注射均对血清皮质酮水平无显著影响。尽管目前关于产前酒精暴露后催乳素水平基线下降的结果尚不清楚，但总体结果表明，产前酒精暴露会降低催乳素水平，但没有证据表明其通过改变雌性大鼠下丘脑的多巴胺能张力来实现这一作用。
