Neidhart M
Department of Rheumatology, University Hospital, Zurich, Switzerland.
Ann Hematol. 1997 Oct;75(4):155-9. doi: 10.1007/s002770050334.
The expected association between age and the CD45 isoforms expression on CD4+ T-PBL is much more obvious in men than in women. We investigated whether or not circulating factors influence the differentiation of CD4+ T-PBL. Peripheral blood samples were obtained from 56 healthy age-matched subjects (28 men and 28 women, 21-55 years old). Mononuclear leukocytes were analyzed by three-color flow cytometry. The serum concentrations of interleukin-1 beta (IL-1 beta), interleukin-6, tumor necrosis factor-alpha (TNF-alpha), GM colony-stimulating factor, prolactin (Prl), and luteinizing hormone (LH) were determined by ELISA. The expected age-related decrease of naive (CD45RA+,RO-) cells and increase of memory (CD45RA-,RO+) cells among CD4+ T-PBL were observed in men only (p < 0.001 and 0.005). In women, these correlations were not significant. On the other hand, in women only, elevated IL-1 beta was associated with fewer naive and more memory cells among CD4+ T-PBL (p < 0.001). In both sexes, IL-1 beta correlated with the expression of CD25 on CD4+ T-PBL (on either naive or memory cells, p < 0.001). Other cytokines or the CD8+ T-PBL showed no significant correlation. In women, the elevation of LH at mid-cycle inversely correlated with the proportion of naive CD4+ T-PBL (p < 0.01). Elevated LH was associated with more CD25 on memory CD4+ T-PBL (p < 0.01). A significant correlation exists between IL-1 beta and LH (p < 0.001). Furthermore, in both sexes, Prl correlated with the proportion of CD4+ cells among T-PBL. In men, elevated Prl was associated with more naive CD4+ T-PBL (p < 0.005), while in women, Prl correlated with more transient CD45RA+, RO+ cells among CD4+ T-PBL and increased TNF-alpha (p < 0.05 for both). Thus, circulating IL-1 beta could be involved in the expression of CD25 on CD4+ T-PBL and favors the generation of memory CD4+ T-PBL. In women, the IL-1 beta- and/or mid-cycle-dependent processes seem to overwhelm the age-related changes. Elevated Prl might exert a dual influence: it favors the development of naive CD4+ T lymphocytes and possibly acts in, synergy with other cytokines during immune stimulation.
年龄与CD4⁺外周血淋巴细胞（T-PBL）上CD45异构体表达之间的预期关联在男性中比在女性中更为明显。我们研究了循环因子是否会影响CD4⁺ T-PBL的分化。从56名年龄匹配的健康受试者（28名男性和28名女性，年龄在21至55岁之间）采集外周血样本。通过三色流式细胞术分析单核白细胞。采用酶联免疫吸附测定法（ELISA）测定血清中白细胞介素-1β（IL-1β）、白细胞介素-6、肿瘤坏死因子-α（TNF-α）、粒细胞-巨噬细胞集落刺激因子、催乳素（Prl）和促黄体生成素（LH）的浓度。仅在男性中观察到CD4⁺ T-PBL中幼稚（CD45RA⁺，RO⁻）细胞数量随年龄增长而减少以及记忆（CD45RA⁻，RO⁺）细胞数量增加（p < 0.001和0.005）。在女性中，这些相关性不显著。另一方面，仅在女性中，IL-1β升高与CD4⁺ T-PBL中较少的幼稚细胞和较多的记忆细胞相关（p < 0.001）。在两性中，IL-1β与CD4⁺ T-PBL上CD25的表达相关（在幼稚细胞或记忆细胞上，p < 0.001）。其他细胞因子或CD8⁺ T-PBL未显示出显著相关性。在女性中，月经周期中期LH升高与幼稚CD4⁺ T-PBL的比例呈负相关（p < 0.01）。LH升高与记忆CD4⁺ T-PBL上更多的CD25相关（p < 0.01）。IL-1β与LH之间存在显著相关性（p < 0.001）。此外，在两性中，Prl与T-PBL中CD4⁺细胞的比例相关。在男性中，Prl升高与更多的幼稚CD4⁺ T-PBL相关（p < 0.005），而在女性中，Prl与CD4⁺ T-PBL中更多的短暂性CD45RA⁺、RO⁺细胞以及TNF-α升高相关（两者均p < 0.05）。因此，循环中的IL-1β可能参与CD4⁺ T-PBL上CD25的表达，并有利于记忆性CD4⁺ T-PBL的产生。在女性中，IL-1β和/或月经周期中期相关过程似乎掩盖了与年龄相关的变化。升高的Prl可能发挥双重作用：它有利于幼稚CD4⁺ T淋巴细胞的发育，并可能在免疫刺激过程中与其他细胞因子协同作用。
