Hopf U, Meyer zum Büschenfelde K H, Hütteroth T
Klin Wochenschr. 1976 Jun 15;54(12):591-8. doi: 10.1007/BF01619575.
For the induction of experimental chronic active hepatitis (CAH) in rabbits long term immunization was performed with human liver-specific protein plus complete Freunds adjuvants. Serum antibodies against allogeneic hepatocellular membrane antigens could be detected after 23 weeks using isolated normal rabbit hepatocytes or by the passive hemagglutination technique with liver-specific membrane protein as antigen. Isolated hepatocytes from these rabbits showed in vivo fixed IgG on their plasma membranes in a linear and granular fluorescence pattern. Furthermore immune complexes (Raji-cell test) were detectable in the sera of these animals. After 23 weeks 3 out of 16 animals had developed a chronic active hepatitis (CAH). After 44 weeks 10 of 15 animals and after 78 weeks 6 of 7 surviving animals had a CAH. All these 7 animals had serum antibodies against membrane antigens of isolated rabbit hepatocytes and in vivo fixed IgG on their hepatocellular plasma membranes. The results suggest the existence of autoantibodies against hepatocellular membrane antigens. In addition, immune complexes may be bound to the plasma membranes. The pathogenetic role of these humoral immune reactions has yet to be determined.
为在兔中诱导实验性慢性活动性肝炎（CAH），用人肝特异性蛋白加完全弗氏佐剂进行长期免疫。23周后，使用分离的正常兔肝细胞或通过以肝特异性膜蛋白为抗原的被动血凝技术，可检测到针对同种异体肝细胞膜抗原的血清抗体。这些兔分离的肝细胞在其质膜上显示出体内固定的IgG，呈线性和颗粒状荧光模式。此外，在这些动物的血清中可检测到免疫复合物（Raji细胞试验）。23周后，16只动物中有3只发生了慢性活动性肝炎（CAH）。44周后，15只动物中有10只，78周后，7只存活动物中有6只患有CAH。所有这7只动物均具有针对分离的兔肝细胞的膜抗原的血清抗体，并且在其肝细胞质膜上有体内固定的IgG。结果提示存在针对肝细胞膜抗原的自身抗体。此外，免疫复合物可能结合到质膜上。这些体液免疫反应的致病作用尚待确定。
