生物繁殖大鼠唾液腺中单核炎性细胞的表型特征

Cohen R E, Talarico G, Noble B

Department of Periodontology, School of Dental Medicine, University at Buffalo, NY 14214, USA.

Arch Oral Biol. 1997 Sep;42(9):649-55. doi: 10.1016/s0003-9969(97)00056-3.

The purpose of this study was to assess whether mononuclear cell abnormalities exist in salivary glands from autoimmune Bio-Breeding (BB) rats. Frozen sections of gland tissues were prepared from five diabetes-resistant BB rats (BB-DR), from five BB rats with diabetes (BB-DP) and from five Wistar rats. A panel of six monoclonal antibodies was used to identify membrane antigens associated primarily with monocytes (ED1), mature tissue macrophages (ED2), lymphoid macrophages (ED3), MHC class II (Ia) antigen (OX6), CD5+ T lymphocytes (OX19), and rat B lymphocytes (OX33). Normal submandibular, sublingual and parotid glands contained few ED1-positive cells, usually two or fewer per field. Tissue macrophages identified by clone ED2 comprised a major mononuclear cell subset in both Wistar and BB rats. However, the number of ED2-positive mononuclear cells was significantly depressed in the submandibular and parotid glands from BB-DR and BB-DP animals, being present in quantities 25-50% of those observed in glands from normal Wistar rats (p < 0.001). In contrast, 25- to 30-fold greater numbers of ED3-positive macrophages were observed in submandibular glands from BB rats (p < 0.001). MHC class II (Ia) antigen expression also was 4- to 6-fold greater in BB rat submandibular glands, compared to Wistar rats (p < 0.001). CD5+ T-lymphocytes were rare or entirely absent in BB sublingual glands (0 to 1 cell per 0.87 mm2 field), compared to 47 cells per field from Wistar sublingual glands. No B lymphocytes were identified with antibody OX33 in any of the rat strains. These findings indicate that BB rat salivary glands differ significantly from Wistar salivary glands. In BB rats there is a rich population of ED3-positive macrophages and T lymphocytes in submandibular gland, low quantities of T lymphocytes in sublingual gland, and fewer ED2-positive macrophages in all three major salivary glands. These differences in mononuclear cell subpopulations may also influence salivary gland function in mucosal immunity.

本研究的目的是评估自身免疫性Bio-Breeding（BB）大鼠的唾液腺中是否存在单核细胞异常。从五只抗糖尿病BB大鼠（BB-DR）、五只患糖尿病的BB大鼠（BB-DP）和五只Wistar大鼠制备腺体组织的冰冻切片。使用一组六种单克隆抗体来识别主要与单核细胞（ED1）、成熟组织巨噬细胞（ED2）、淋巴样巨噬细胞（ED3）、MHC II类（Ia）抗原（OX6）、CD5⁺ T淋巴细胞（OX19）和大鼠B淋巴细胞（OX33）相关的膜抗原。正常下颌下腺、舌下腺和腮腺中ED1阳性细胞很少，通常每个视野两个或更少。克隆ED2识别的组织巨噬细胞在Wistar大鼠和BB大鼠中均构成主要的单核细胞亚群。然而，BB-DR和BB-DP动物下颌下腺和腮腺中ED2阳性单核细胞的数量显著减少，其数量仅为正常Wistar大鼠腺体中观察到数量的25%-50%（p<0.001）。相反，在BB大鼠的下颌下腺中观察到ED3阳性巨噬细胞的数量比正常多25至30倍（p<0.001）。与Wistar大鼠相比，BB大鼠下颌下腺中MHC II类（Ia）抗原的表达也高4至6倍（p<0.001）。与Wistar大鼠舌下腺每个视野47个细胞相比，BB大鼠舌下腺中CD5⁺ T淋巴细胞很少或完全不存在（每0.87平方毫米视野0至1个细胞）。在任何大鼠品系中均未用抗体OX33识别出B淋巴细胞。这些发现表明，BB大鼠的唾液腺与Wistar大鼠的唾液腺有显著差异。在BB大鼠中，下颌下腺有丰富的ED3阳性巨噬细胞和T淋巴细胞，舌下腺中T淋巴细胞数量少，并且在所有三个主要唾液腺中ED2阳性巨噬细胞较少。单核细胞亚群的这些差异也可能影响黏膜免疫中的唾液腺功能。