Cohen R E, Noble B, Neiders M E, Bedi G S, Comeau R L
Department of Periodontology, School of Medicine, State University of New York, Buffalo 14214.
Arch Oral Biol. 1992;37(6):503-9. doi: 10.1016/0003-9969(92)90107-j.
Macrophages exert a major effect in the stimulation of lymphocytes and the modulation of immunological responses. To determine the presence and phenotypic distribution of the resident cells of the mononuclear phagocyte system in submandibular glands, frozen sections were prepared from five normal rats, and from six rats treated with 20 mg/kg isoproterenol/day for 10 days. A panel of six monoclonal antibodies was used to identify membrane markers associated primarily with circulatory monocytes (ED1), mature tissue macrophages (ED2), lymphoid macrophages (ED3), Ia antigen (OX6), CD5-positive T lymphocytes (OX19) and rat B lymphocytes (OX33). Cells identified by each monoclonal antibody were quantified by averaging the number of positive cells in 10 consecutive random high-power fields. ED2 cells (165 cells/field) were predominant in normal rat submandibular gland, followed by lower numbers of OX6-positive cells (18 cells/field). Cells positive for the remaining markers were also present in smaller amounts. In submandibular glands, treatment of rats with isoproterenol resulted in an increase in ED1-positive cells (from 2 to 39 cells/field), but also in substantial decreases in the number of cells positive for the remaining cell markers. B cells were not detected in any of the submandibular glands examined. These data suggest that isoproterenol induces a mild inflammatory response within rat submandibular glands that is not observed in normal glands. This results in an increase in the relative number of infiltrating monocytes compared to the number of more mature tissue macrophages.
巨噬细胞在刺激淋巴细胞和调节免疫反应方面发挥着主要作用。为了确定下颌下腺中单核吞噬细胞系统驻留细胞的存在及表型分布，我们从五只正常大鼠以及六只每天接受20mg/kg异丙肾上腺素治疗10天的大鼠身上制备了冰冻切片。使用一组六种单克隆抗体来识别主要与循环单核细胞（ED1）、成熟组织巨噬细胞（ED2）、淋巴样巨噬细胞（ED3）、Ia抗原（OX6）、CD5阳性T淋巴细胞（OX19）和大鼠B淋巴细胞（OX33）相关的膜标记物。通过对10个连续随机高倍视野中的阳性细胞数量进行平均，对每种单克隆抗体识别的细胞进行定量。ED2细胞（165个细胞/视野）在正常大鼠下颌下腺中占主导地位，其次是数量较少的OX6阳性细胞（18个细胞/视野）。其余标记物阳性的细胞数量也较少。在下颌下腺中，用异丙肾上腺素处理大鼠导致ED1阳性细胞增加（从2个细胞/视野增加到39个细胞/视野），但其余细胞标记物阳性细胞的数量也大幅减少。在所检查的任何下颌下腺中均未检测到B细胞。这些数据表明，异丙肾上腺素在大鼠下颌下腺中诱导了一种正常腺体中未观察到的轻度炎症反应。这导致浸润单核细胞的相对数量相较于更成熟的组织巨噬细胞数量增加。
