Neely M D, Macaluso E
Department of Pharmacology, University of Basel, Switzerland.
Proc Biol Sci. 1997 Nov 22;264(1388):1701-6. doi: 10.1098/rspb.1997.0236.
Cell motility is produced by changes in the dynamics and organization of actin filaments. The aim of the experiments described here was to test whether growing neurites contain two actin-binding proteins, gelsolin and profilin, that regulate polymerization of actin and affect non-neuronal cell motility. The distribution of gelsolin, profilin and the microfilaments was compared by immunocytochemistry of leech neurons growing in culture. We observed that microfilaments are enriched in the peripheral motile areas of the neurites. Both gelsolin and profilin are also concentrated in these regions. Gelsolin is abundant in filopodia and is associated with single identifiable microfilament bundles in lamellipodia. Profilin is not prominent in filopodia and shows a diffuse staining pattern in lamellipodia. The colocalization of gelsolin and profilin in motile, microfilament-rich areas supports the hypothesis that they synergistically regulate the actin dynamics that underlie neurite growth.
细胞运动是由肌动蛋白丝的动力学和组织变化产生的。本文所述实验的目的是测试生长中的神经突是否含有两种肌动蛋白结合蛋白，凝溶胶蛋白和前纤维蛋白，它们调节肌动蛋白的聚合并影响非神经元细胞的运动。通过对培养中的水蛭神经元进行免疫细胞化学，比较了凝溶胶蛋白、前纤维蛋白和微丝的分布。我们观察到微丝在神经突的外周运动区域富集。凝溶胶蛋白和前纤维蛋白也都集中在这些区域。凝溶胶蛋白在丝状伪足中丰富，并与片状伪足中单个可识别的微丝束相关。前纤维蛋白在丝状伪足中不突出，在片状伪足中呈弥漫性染色模式。凝溶胶蛋白和前纤维蛋白在富含微丝的运动区域共定位，支持了它们协同调节神经突生长基础的肌动蛋白动力学的假设。
