鼠模型中的实验性克氏锥虫感染

Experimental Trypanosoma rangeli infection in a murine model.

作者信息

Horna A E, Saldaña A, Orn A, Sousa O E

机构信息

Centro de Investigación y Diagnóstico de Enfermedades Parasitarias (CIDEP), Universidad de Panamá, Panamá.

出版信息

Rev Biol Trop. 1997 Mar;44-45:125-9.

PMID:9404516

Abstract

Trypanosoma rangeli experimental murine infections were performed in order to study parasitemias and anti-parasite antibody levels. Three groups of mice were used: a) mice infected with metatrypomastigotes derived from infected bugs; b) mice which received four reinoculations of metatrypomastigotes and c) mice immunosuppressed with cyclophosphamide. The results showed that bloodstream parasites can be found from the first day post inoculation reaching a peak at day 5 or 7 and then start to decline. Parasites disappeared completely from the circulation after 20-25 days. However in the immunosuppressed group, parasites were found in blood up to 45 days post infection. The humoral immune response was monitored using an ELISA test and low levels of specific IgG and IgM immunoglobulins were found. However the IgG titers were lower than the IgM. One could conclude that IgM was the predominant immunoglobulin isotype induced in a T. rangeli experimental infection because the highest titers were observed in the reinoculated group. IgM antibodies also showed the most prominent crossreactivities with T. cruzi antigens.

摘要

为了研究寄生虫血症和抗寄生虫抗体水平，进行了克氏锥虫实验性小鼠感染实验。使用了三组小鼠：a）感染了来自受感染昆虫的后循环锥鞭毛体的小鼠；b）接受四次后循环锥鞭毛体再接种的小鼠；c）用环磷酰胺免疫抑制的小鼠。结果表明，接种后第一天即可在血液中发现寄生虫，在第5天或第7天达到峰值，然后开始下降。感染后20 - 25天，寄生虫从循环系统中完全消失。然而，在免疫抑制组中，感染后45天仍可在血液中发现寄生虫。使用酶联免疫吸附测定（ELISA）试验监测体液免疫反应，发现特异性IgG和IgM免疫球蛋白水平较低。然而，IgG滴度低于IgM。可以得出结论，IgM是克氏锥虫实验性感染中诱导产生的主要免疫球蛋白同种型，因为在再接种组中观察到了最高滴度。IgM抗体与克氏锥虫抗原也表现出最显著的交叉反应性。