LaLonde C, Nayak U, Hennigan J, Demling R
Beth Israel Deaconess Hospital, Boston, Massachusetts, USA.
J Burn Care Rehabil. 1997 Nov-Dec;18(6):515-9. doi: 10.1097/00004630-199711000-00008.
We determined the effect of a severe smoke exposure on plasma oxidant and antioxidant activity. Adult sheep were given a smoke exposure while under anesthesia that produced a carboxyhemoglobin level of 45% +/- 3%. Twelve sheep were studied; six were given smoke alone and volume-resuscitated with sufficient lactated Ringer's solution to maintain baseline hemodynamics. This response was compared with six control sheep during a 6-hour period. The smoke inhalation injury produced a significant increase in plasma hydrogen peroxide and a significant decrease in plasma lipid peroxidation. Circulating lipid peroxidation did not correlate with tissue lipid peroxidation because lung and liver lipid peroxidation were significantly increased. The plasma antioxidants glutathione, catalase, and vitamin E were significantly reduced in response to the injury. Vitamin C remained unchanged from control. Circulatory failure is not a key element in this study, because lactate levels were controlled with volume resuscitation. The degree of smoke inhalation to the airway produced distant organ lipid peroxidation and a decrease in circulating antioxidants--without producing an increase in circulating lipid peroxidation. Maintaining circulating antioxidants may prevent distant organ lipid peroxidation and may be of clinical use in devising treatment strategies for smoke inhalation injury with the availability of antioxidants.
我们测定了严重烟雾暴露对血浆氧化剂和抗氧化剂活性的影响。成年绵羊在麻醉状态下接受烟雾暴露，使其碳氧血红蛋白水平达到45%±3%。对12只绵羊进行了研究；其中6只仅接受烟雾暴露，并用足量的乳酸林格氏液进行容量复苏以维持基线血流动力学。在6小时内，将这种反应与6只对照绵羊进行了比较。烟雾吸入损伤导致血浆过氧化氢显著增加，血浆脂质过氧化显著降低。循环脂质过氧化与组织脂质过氧化不相关，因为肺和肝脂质过氧化显著增加。损伤导致血浆抗氧化剂谷胱甘肽、过氧化氢酶和维生素E显著减少。维生素C与对照相比保持不变。循环衰竭不是本研究的关键因素，因为通过容量复苏控制了乳酸水平。气道烟雾吸入的程度导致远处器官脂质过氧化和循环抗氧化剂减少——而循环脂质过氧化未增加。维持循环抗氧化剂可能预防远处器官脂质过氧化，并且在有抗氧化剂可用的情况下，对于制定烟雾吸入损伤的治疗策略可能具有临床用途。
