Liu Y H, Fujitani N, Koda Y, Kimura H
Division of Human Genetics, Department of Forensic Medicine, Kurume University School of Medicine, Kurume, Fukuoka, Japan.
J Histochem Cytochem. 1998 Jan;46(1):69-76. doi: 10.1177/002215549804600109.
We have examined the immunohistochemical distribution of H Type 1 and of H Type 2 substances of the ABO blood group system in human submandibular gland using either of the two anti-H monoclonal antibodies MAb 1E3 and MAb 3A5. MAb 3A5 was specific for H Type 2, and MAb 1E3 reacted with each of H Type 1-H Type 4 artificial antigens. We have developed a competitive inhibition method against H Type 2 and have obtained MAb 1E3, which is fairly specific for H Type 1 under certain conditions. Mucous cells from secretors were strongly stained by 1E3 and weakly by 3A5, whereas those from nonsecretors showed no reaction with 1E3 and 3A5. Serous cells from both secretors and nonsecretors were stained neither by 1E3 nor by 3A5. Striated and interlobular duct cells were strongly stained by 1E3 and by 3A5, regardless of the secretor status. These results indicated that the expressions of the H Type 1 and H Type 2 in different cell types of the submandibular gland were controlled by different genes. In addition, we have determined the acceptor specificity of two alpha(1,2)fucosyltransferases (H and Se enzymes) after transient expressions of the FUT1 and FUT2 in COS7 cells, and found that the H enzyme activity was similar for both Type 1 and Type 2 precursors, and that Se enzyme activity with the Type 1 precursor was higher than that with the Type 2 precursor. Expression of the H Type 1 antigen in mucous cells was found to be dependent on the Se gene, whereas expressions of the H Type 1 and H Type 2 antigens in striated and interlobular duct cells were dependent on the H gene. (J Histochem Cytochem 46:69-76, 1998)
我们使用两种抗-H单克隆抗体MAb 1E3和MAb 3A5中的任何一种，研究了ABO血型系统中H1型和H2型物质在人类下颌下腺中的免疫组织化学分布。MAb 3A5对H2型具有特异性，而MAb 1E3与H1型至H4型人工抗原中的每一种都发生反应。我们开发了一种针对H2型的竞争性抑制方法，并获得了在某些条件下对H1型相当特异的MAb 1E3。分泌者的黏液细胞被1E3强烈染色，被3A5弱染色，而非分泌者的黏液细胞与1E3和3A5均无反应。分泌者和非分泌者的浆液细胞均未被1E3和3A5染色。纹状管和小叶间导管细胞被1E3和3A5强烈染色，与分泌状态无关。这些结果表明，下颌下腺不同细胞类型中H1型和H2型的表达受不同基因控制。此外，我们在COS7细胞中瞬时表达FUT1和FUT2后，确定了两种α(1,2)岩藻糖基转移酶（H和Se酶）的受体特异性，发现H酶对1型和2型前体的活性相似，且Se酶对1型前体的活性高于对2型前体的活性。发现黏液细胞中H1型抗原的表达依赖于Se基因，而纹状管和小叶间导管细胞中H1型和H2型抗原的表达依赖于H基因。（《组织化学与细胞化学杂志》46:69 - 76, 1998）
