构巢曲霉（= 烟曲霉）基因组的体外重建。
In vitro reconstruction of the Aspergillus (= Emericella) nidulans genome.
作者信息
Prade R A, Griffith J, Kochut K, Arnold J, Timberlake W E
机构信息
Department of Microbiology and Molecular Genetics, Oklahoma State University, Stillwater, OK 74078-0289, USA.
出版信息
Proc Natl Acad Sci U S A. 1997 Dec 23;94(26):14564-9. doi: 10.1073/pnas.94.26.14564.
Abstract
A physical map of the 31-megabase Aspergillus nidulans genome is reported, in which 94% of 5,134 cosmids are assigned to 49 contiguous segments. The physical map is the result of a two-way ordering process, in which clones and probes were ordered simultaneously on a binary DNA/DNA hybridization matrix. Compression by elimination of redundant clones resulted in a minimal map, which is a chromosome walk. Repetitive DNA is nonrandomly dispersed in the A. nidulans genome, reminiscent of heterochromatic banding patterns of higher eukaryotes. We hypothesize gene clusters may arise by horizontal transfer and spread by transposition to explain the nonrandom pattern of repeats along chromosomes.
摘要
报道了31兆碱基的构巢曲霉基因组的物理图谱，其中5134个黏粒中的94%被定位到49个连续片段上。该物理图谱是双向排序过程的结果，在这个过程中，克隆和探针在二元DNA/DNA杂交矩阵上同时进行排序。通过消除冗余克隆进行压缩得到了一个最小图谱，这是一个染色体步移。重复DNA在构巢曲霉基因组中呈非随机分布，这让人联想到高等真核生物的异染色质带型。我们推测基因簇可能通过水平转移产生，并通过转座传播，以解释沿着染色体的重复序列的非随机模式。
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