Buades C, Moya A
Departamento de Genética, Facultad de Biología, Universidad de Valencia, Spain.
J Mol Evol. 1996 May;42(5):537-42. doi: 10.1007/BF02352283.
A phylogenetic study of the isopenicillin-N-synthetase (IPNS) gene sequence from prokaryotic and lower eukaryotic producers of beta-lactam antibiotics by means of a maximum-likelihood approach has been carried out. After performing an extensive search, rather than invoking a global molecular clock, the results obtained are best explained by a model with three rates of evolution. Grouped in decreasing order, these correspond to A. nidulans and then to the rest of the eukaryotes and prokaryotes, respectively. The estimated branching date between prokaryotic and fungal IPNS sequences (852 +/- 106 MY) strongly supports the hypothesis that the IPNS gene was horizontally transferred from bacterial beta-lactam producers to filamentous fungi.
通过最大似然法对β-内酰胺抗生素的原核和低等真核生物生产者的异青霉素N合成酶（IPNS）基因序列进行了系统发育研究。在进行广泛搜索后，并非采用全局分子钟，而是用具有三种进化速率的模型能最好地解释所得结果。按降序排列，这些速率分别对应构巢曲霉，然后是其他真核生物和原核生物。原核生物和真菌IPNS序列之间估计的分支日期（852±106百万年）有力地支持了IPNS基因从细菌β-内酰胺生产者水平转移到丝状真菌的假说。
