WIP是一种与威斯科特-奥尔德里奇综合征蛋白相关的蛋白质，可诱导淋巴细胞中的肌动蛋白聚合和重新分布。
WIP, a protein associated with wiskott-aldrich syndrome protein, induces actin polymerization and redistribution in lymphoid cells.
作者信息
Ramesh N, Antón I M, Hartwig J H, Geha R S
机构信息
Division of Immunology, Children's Hospital, Harvard Medical School, Boston, MA 02115, USA.
出版信息
Proc Natl Acad Sci U S A. 1997 Dec 23;94(26):14671-6. doi: 10.1073/pnas.94.26.14671.
Abstract
Wiskott-Aldrich syndrome (WAS) is an X-linked immunodeficiency caused by mutations that affect the WAS protein (WASP) and characterized by cytoskeletal abnormalities in hematopoietic cells. By using the yeast two-hybrid system we have identified a proline-rich WASP-interacting protein (WIP), which coimmunoprecipitated with WASP from lymphocytes. WIP binds to WASP at a site distinct from the Cdc42 binding site and has actin as well as profilin binding motifs. Expression of WIP in human B cells, but not of a WIP truncation mutant that lacks the actin binding motif, increased polymerized actin content and induced the appearance of actin-containing cerebriform projections on the cell surface. These results suggest that WIP plays a role in cortical actin assembly that may be important for lymphocyte function.
摘要
威斯科特-奥尔德里奇综合征（WAS）是一种X连锁免疫缺陷病，由影响威斯科特-奥尔德里奇综合征蛋白（WASP）的突变引起，其特征为造血细胞中的细胞骨架异常。通过使用酵母双杂交系统，我们鉴定出一种富含脯氨酸的WASP相互作用蛋白（WIP），它可与淋巴细胞中的WASP进行共免疫沉淀。WIP在一个不同于Cdc42结合位点的部位与WASP结合，并且具有肌动蛋白以及肌动蛋白结合蛋白结合基序。WIP在人B细胞中的表达增加了聚合肌动蛋白的含量，并诱导细胞表面出现含肌动蛋白的脑回样突起，但缺乏肌动蛋白结合基序的WIP截短突变体则没有这种作用。这些结果表明，WIP在皮层肌动蛋白组装中发挥作用，这可能对淋巴细胞功能很重要。
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本文引用的文献
1
Pillars Article: Isolation of a Novel Gene Mutated in Wiskott-Aldrich Syndrome. . 1994. 78: 635-644.支柱文章：威斯科特-奥尔德里奇综合征中一个新的突变基因的分离。. 1994年。78卷：635 - 644页。
J Immunol. 2018 Jun 1;200(11):3671-3680.
2
ABM-1 and ABM-2 homology sequences: consensus docking sites for actin-based motility defined by oligoproline regions in Listeria ActA surface protein and human VASP.ABM-1和ABM-2同源序列：由李斯特菌ActA表面蛋白和人类血管舒张刺激磷蛋白中的寡脯氨酸区域定义的基于肌动蛋白运动的共有对接位点。
Biochem Biophys Res Commun. 1997 Feb 24;231(3):686-91. doi: 10.1006/bbrc.1997.6158.
3
The many faces of WAS protein.WAS蛋白的多种面貌。
Science. 1997 Jan 3;275(5296):27-8. doi: 10.1126/science.275.5296.27.
4
Association of human fas (CD95) with a ubiquitin-conjugating enzyme (UBC-FAP).人类Fas（CD95）与泛素结合酶（UBC-FAP）的关联。
J Biol Chem. 1996 Dec 6;271(49):31037-43. doi: 10.1074/jbc.271.49.31037.
5
6
Mena, a relative of VASP and Drosophila Enabled, is implicated in the control of microfilament dynamics.Mena是VASP的亲属，与果蝇Enabled相关，参与微丝动力学的调控。
Cell. 1996 Oct 18;87(2):227-39. doi: 10.1016/s0092-8674(00)81341-0.
7
Identification of regions of the Wiskott-Aldrich syndrome protein responsible for association with selected Src homology 3 domains.确定威斯科特-奥尔德里奇综合征蛋白中与选定的Src同源3结构域相关的区域。
J Biol Chem. 1996 Oct 18;271(42):26291-5. doi: 10.1074/jbc.271.42.26291.
8
Identification of Itk/Tsk Src homology 3 domain ligands.Itk/Tsk Src同源结构域3配体的鉴定。
J Biol Chem. 1996 Oct 11;271(41):25646-56. doi: 10.1074/jbc.271.41.25646.
9
Wiskott-Aldrich syndrome protein (WASp) is a binding partner for c-Src family protein-tyrosine kinases.威斯科特-奥尔德里奇综合征蛋白（WASp）是c-Src家族蛋白酪氨酸激酶的结合伴侣。
Curr Biol. 1996 Aug 1;6(8):981-8. doi: 10.1016/s0960-9822(02)00642-5.
10
Two GTPases, Cdc42 and Rac, bind directly to a protein implicated in the immunodeficiency disorder Wiskott-Aldrich syndrome.两种鸟苷三磷酸酶，即Cdc42和Rac，直接与一种与免疫缺陷疾病威斯科特-奥尔德里奇综合征相关的蛋白质结合。
Curr Biol. 1996 Jan 1;6(1):70-5. doi: 10.1016/s0960-9822(02)00423-2.
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