Brüstle O, Choudhary K, Karram K, Hüttner A, Murray K, Dubois-Dalcq M, McKay R D
Laboratory of Molecular Biology, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD 20892-4092, USA.
Nat Biotechnol. 1998 Nov;16(11):1040-4. doi: 10.1038/3481.
Limited experimental access to the central nervous system (CNS) is a key problem in the study of human neural development, disease, and regeneration. We have addressed this problem by generating neural chimeras composed of human and rodent cells. Fetal human brain cells implanted into the cerebral ventricles of embryonic rats incorporate individually into all major compartments of the brain, generating widespread CNS chimerism. The human cells differentiate into neurons, astrocytes, and oligodendrocytes, which populate the host fore-, mid-, and hindbrain. These chimeras provide a unique model to study human neural cell migration and differentiation in a functional nervous system.
在人类神经发育、疾病和再生研究中，对中枢神经系统（CNS）进行有限的实验性研究是一个关键问题。我们通过生成由人类和啮齿动物细胞组成的神经嵌合体来解决这个问题。植入胚胎大鼠脑室的胎儿人类脑细胞会分别融入大脑的所有主要区域，产生广泛的中枢神经系统嵌合现象。人类细胞分化为神经元、星形胶质细胞和少突胶质细胞，这些细胞分布在宿主的前脑、中脑和后脑。这些嵌合体为研究功能性神经系统中人类神经细胞的迁移和分化提供了一个独特的模型。
