血清淀粉样蛋白A/SAA4组成型同种型的自动测量及其与其他载脂蛋白的比较。
Automated measurement of a constitutive isotype of serum amyloid A/SAA4 and comparison with other apolipoproteins.
作者信息
Yamada T, Wada A, Yamaguchi T, Itoh Y, Kawai T
机构信息
Department of Clinical Pathology, Jichi Medical School, Tochigi, Japan.
出版信息
J Clin Lab Anal. 1997;11(6):363-8. doi: 10.1002/(SICI)1098-2825(1997)11:6<363::AID-JCLA10>3.0.CO;2-U.
A constitutive isotype of serum amyloid A (SAA4) is present mostly in high density lipoprotein (HDL) and a little in other lipoproteins. In this study, we developed an automated method for measuring SAA4 concentration in serum by kinetic nephelometry of anti-SAA4 antibody-coated latex agglutination. Rabbit antibodies generated by immunization of recombinant SAA4 were found to have no apparent reactivity with acute phase SAA. The values determined by this method and by the previous enzyme immunoassay showed good agreement (r = 0.862). Serum SAA4 values of 26 healthy adults ranged from 37-109 mg/L (mean; 62 mg/L). Their SAA4 concentrations were not significantly related with those of apolipoprotein A-I, A-II, B, C-II, C-III or E. Also, SAA4 did not correlate with cholesterol in preparation after removal of very low density lipoprotein and low density lipoprotein. These suggest the unique behavior of SAA4 in lipoprotein metabolism, while what contributes to variation of SAA4 levels in serum, especially in HDL, remains to be clarified.
血清淀粉样蛋白A的组成型同种型（SAA4）主要存在于高密度脂蛋白（HDL）中，在其他脂蛋白中含量较少。在本研究中，我们开发了一种通过抗SAA4抗体包被的乳胶凝集动力学散射比浊法自动测定血清中SAA4浓度的方法。经重组SAA4免疫产生的兔抗体与急性期SAA无明显反应性。用该方法和先前的酶免疫测定法测定的值显示出良好的一致性（r = 0.862）。26名健康成年人的血清SAA4值范围为37 - 109 mg/L（平均值；62 mg/L）。他们的SAA4浓度与载脂蛋白A-I、A-II、B、C-II、C-III或E的浓度无显著相关性。此外，在去除极低密度脂蛋白和低密度脂蛋白后，制备物中的SAA4与胆固醇也无相关性。这些表明SAA4在脂蛋白代谢中的独特行为，而导致血清中，尤其是HDL中SAA4水平变化的原因仍有待阐明。
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