Yamada T, Nomata Y, Sugita O, Okada M
Department of Laboratory Medicine, Niigata University School of Medicine, Niigata, Japan.
Ann Clin Biochem. 1993 Jan;30 ( Pt 1):72-6. doi: 10.1177/000456329303000112.
Serum amyloid A protein (SAA) is a sensitive acute phase reactant. We developed a method for the rapid measurement of human SAA in serum by kinetic nephelometry of anti-SAA antibody-coated latex agglutination. Measurement takes less than 6 min using an automated analyser. Standardization of the assay employs SAA-enriched high density lipoprotein as the primary standard. The values determined by our new method and by conventional enzyme-immunoassay showed good agreement (r = 0.988). The normal range was 0.17-10.0 mg/L [mean(SD)]. This rapid method should prove useful in clinical laboratories.
血清淀粉样蛋白A（SAA）是一种敏感的急性期反应物。我们开发了一种通过抗SAA抗体包被的乳胶凝集动力学散射比浊法快速测定血清中人类SAA的方法。使用自动分析仪测量时间不到6分钟。该检测方法的标准化采用富含SAA的高密度脂蛋白作为主要标准品。我们的新方法和传统酶免疫法测定的值显示出良好的一致性（r = 0.988）。正常范围为0.17 - 10.0 mg/L [平均值（标准差）]。这种快速方法在临床实验室中应会证明是有用的。
