Daste G, Gioanni J, Lauque D, Kremps M, Mazeau C, Paquis P, Schneider M
Laboratoire d'Anatomie et Cytologie Pathologiques, CHU Purpan, Toulouse, France.
Arch Anat Cytol Pathol. 1997;45(4):185-91.
Monoclonal antibody GC12 was examined for its value in cytopathologic diagnosis. Its sensitivity and specificity in staining reactive mesothelial cells in serous effusions was determined using the indirect immuno alkaline phosphatase anti-alkaline phosphatase technique. Smears prepared from 78 serous effusions (15 benign, 54 malignant, 9 atypical) were immunostained with GC12 and five other monoclonal antibodies: KL1 directed against cytokeratins, D33 against desmin, E29 against epithelial membrane antigen, Calam 27 against epithelial surface antigen and NEO 723 reactive with carcinoembryonic antigen. GC12 reacted positively with 80 to 100% mesothelial cells from the 15 benign effusions. Carcinoma cells were negative in 87% of malignant cases. Ovarian carcinomas were specially stained among positive cases. One malignant mesothelioma was positive and one was unreactive with GC12. Moreover, the reactivity of atypical cells in 9 serous effusions with GC12 was helpful to characterize benign and malignant effusions: the immunophenotype GC12-, Desmine-, Calam 27+, EMA+, ACE+ allowed detection of carcinoma cells in 2 cases. It is concluded that GC12 is a good tool for distinguishing carcinoma cells from reactive mesothelial cells in serous effusions.
检测了单克隆抗体GC12在细胞病理学诊断中的价值。采用间接免疫碱性磷酸酶抗碱性磷酸酶技术，确定其对浆液性积液中反应性间皮细胞染色的敏感性和特异性。用GC12和其他五种单克隆抗体对78份浆液性积液（15份良性、54份恶性、9份非典型）制备的涂片进行免疫染色：KL1针对细胞角蛋白，D33针对结蛋白，E29针对上皮膜抗原，Calam 27针对上皮表面抗原，NEO 723与癌胚抗原反应。GC12与15份良性积液中的80%至100%的间皮细胞呈阳性反应。在87%的恶性病例中癌细胞呈阴性。在阳性病例中卵巢癌有特异性染色。1例恶性间皮瘤呈阳性，1例与GC12无反应。此外，9份浆液性积液中非典型细胞与GC12的反应性有助于鉴别良性和恶性积液：免疫表型GC12-、结蛋白-、Calam 27+、EMA+、ACE+在2例中检测到癌细胞。结论是GC12是区分浆液性积液中癌细胞与反应性间皮细胞的良好工具。
