Wu Q, Storrier G D, Pariente F, Wang Y, Shapleigh J P, Abruña H D
Department of Chemistry, Baker Laboratory, Cornell University, Ithaca, New York 14853, USA.
Anal Chem. 1997 Dec 1;69(23):4856-63. doi: 10.1021/ac970595p.
The preparation and electrochemical characterization of glassy carbon electrodes (GCEs) modified with electropolymerized films of the cation N-(3-pyrrol-1-yl-propyl)-4,4'-bipyridine (PPB) are described. The behavior of a new biosensor, which exhibits a high catalytic activity for nitrite reduction and which consists of a maltose binding protein nitrite reductase fusion (MBP-Nir) immobilized on an electropolymerized film of PPB as an electrocatalyst, is also described. The insoluble perchlorate salt of the poly(benzyl viologen) dication was used to immobilize MBP-Nir onto an electrode previously modified with an electropolymerized film of PPB. The electropolymerized film of PPB on the GCE is redox active and exhibits special electron-transfer properties toward the MBP-Nir layer but not toward Nir (Nir without MBP fusion attached), suggesting an intimate interaction between the PPB film and the MBP-Nir layer. The kinetics of the catalytic reaction between the biosensor and nitrite anion were characterized using cyclic voltammetry and rotated disk electrode techniques, and a value of (4.6 +/- 0.5) x 10(3) M-1 S-1 was obtained for the rate constant.
描述了用阳离子N-(3-吡咯-1-基丙基)-4,4'-联吡啶(PPB)的电聚合膜修饰玻碳电极(GCEs)的制备及其电化学表征。还描述了一种新型生物传感器的行为，该传感器对亚硝酸盐还原具有高催化活性，由固定在PPB电聚合膜上作为电催化剂的麦芽糖结合蛋白亚硝酸还原酶融合体(MBP-Nir)组成。聚(苄基紫精)二价阳离子的不溶性高氯酸盐用于将MBP-Nir固定在先前用PPB电聚合膜修饰的电极上。GCE上的PPB电聚合膜具有氧化还原活性，对MBP-Nir层表现出特殊的电子转移性质，但对Nir(未连接MBP融合体的Nir)则没有，这表明PPB膜与MBP-Nir层之间存在紧密相互作用。使用循环伏安法和旋转圆盘电极技术对生物传感器与亚硝酸根阴离子之间的催化反应动力学进行了表征，得到的速率常数为(4.6±0.5)×10(3)M-1 S-1。
