Tokue Y, Sugano K, Noda T, Saito D, Shimosato Y, Ohkura H, Kakizoe T, Sekiya T
Clinical Laboratory Division, National Cancer Center Hospital, Tokyo, Japan.
Diagn Microbiol Infect Dis. 1995 Dec;23(4):129-33. doi: 10.1016/0732-8893(95)00198-0.
Clinical isolates of mycobacteria were identified to species levels using nonradioisotopic single-strand conformation polymorphism (non-RI SSCP) analysis of 16S rRNA gene fragments amplified by polymerase chain reaction with primers common to all of mycobacterial species. The method is based on a hypervariable region within the 16S rRNA in mycobacteria, which is characterized by species-specific nucleotide sequences. A total of 92 mycobacterial strains (Mycobacterium tuberculosis, M. avium, M. gordonae, M. intracellulare, M. kansasii, M. chelonae, M. nonchromogenicum, M. xenopi, and unidentified strain) were studied. They were classified into nine types of pattern showing single-strand DNA bands having different mobilities. Each strain was shown in the species-specific mobility by non-RI SSCP analysis. The results of non-RI SSCP analysis were identical to those of standard biochemical methods and 16S rRNA sequencing.
利用聚合酶链反应，以所有分枝杆菌属物种通用的引物扩增16S rRNA基因片段，通过非放射性单链构象多态性（non-RI SSCP）分析，将分枝杆菌临床分离株鉴定到种水平。该方法基于分枝杆菌16S rRNA内的一个高变区，其特征在于物种特异性核苷酸序列。共研究了92株分枝杆菌菌株（结核分枝杆菌、鸟分枝杆菌、戈登分枝杆菌、胞内分枝杆菌、堪萨斯分枝杆菌、龟分枝杆菌、非产色分枝杆菌、蟾分枝杆菌和未鉴定菌株）。它们被分为九种类型的图谱，显示出具有不同迁移率的单链DNA条带。通过non-RI SSCP分析，每个菌株都呈现出物种特异性迁移率。non-RI SSCP分析结果与标准生化方法和16S rRNA测序结果一致。
