通过半巢式逆转录聚合酶链反应（seminested RT-PCR）提高鼻洗液中呼吸道合胞病毒的检测率。

Improved detection of respiratory syncytial virus in nasal aspirates by seminested RT-PCR.

作者信息

Henkel J H, Aberle S W, Kundi M, Popow-Kraupp T

机构信息

Institute of Virology, University of Vienna, Austria.

出版信息

J Med Virol. 1997 Dec;53(4):366-71.

PMID:9407385

Abstract

A seminested RT-PCR for amplification of Respiratory syncytial virus (RSV)-RNA in nasal aspirates has been developed and used to test nasopharyngeal aspirates (NPAs) from 132 infants hospitalized with acute respiratory tract infections during winter epidemics. The results were compared with those obtained by virus isolation in tissue culture and antigen detection with an enzyme-linked immunosorbent assay (Ag-ELISA). RSV-RNA was detected by seminested RT-PCR in 57 of the 59 samples that were positive by virus isolation and/or ELISA, as well as in 25 of 73 samples negative by virus isolation and ELISA. Eighteen of these 25 samples were obtained from children older than one year of age, 17 of whom were experiencing reinfection, as indicated by the presence of preexisting serum RSV-IgG antibodies. These results indicate that seminested RT-PCR is more sensitive than conventional methods for the detection of RSV in patients experiencing reinfections and suggest that this assay might also be useful for rapid diagnosis of RSV infections in older people.

摘要

已开发出一种半巢式逆转录聚合酶链反应（RT-PCR），用于扩增鼻吸出物中的呼吸道合胞病毒（RSV）RNA，并用于检测冬季流行期间因急性呼吸道感染住院的132名婴儿的鼻咽吸出物（NPA）。将结果与通过组织培养中的病毒分离和酶联免疫吸附测定（Ag-ELISA）进行抗原检测所获得的结果进行比较。在病毒分离和/或ELISA检测呈阳性的59个样本中，有57个通过半巢式RT-PCR检测到RSV-RNA，在病毒分离和ELISA检测呈阴性的73个样本中，有25个检测到RSV-RNA。这25个样本中有18个取自一岁以上儿童，其中17个存在再感染情况，这可通过预先存在的血清RSV-IgG抗体的存在来表明。这些结果表明，半巢式RT-PCR在检测再感染患者中的RSV方面比传统方法更敏感，并表明该检测方法可能也有助于老年人RSV感染的快速诊断。