Reis Alexanda Dias, Fink Maria Cristina Domingues, Machado Clarisse Martins, Paz José de Paula, Oliveira Renato Reis, Tateno Adriana Fumie, Machado Adriana Freire, Cardoso Maria Regina, Pannuti Claudio Sérgio
Laboratório de Virologia, Instituto de Medicina Tropical de São Paulo, Universidade de São PauloSão Paulo, SP, Brazil.
Rev Inst Med Trop Sao Paulo. 2008 Jan-Feb;50(1):37-40. doi: 10.1590/s0036-46652008000100008.
A total of 316 samples of nasopharyngeal aspirate from infants up to two years of age with acute respiratory-tract illnesses were processed for detection of respiratory syncytial virus (RSV) using three different techniques: viral isolation, direct immunofluorescence, and PCR. Of the samples, 36 (11.4%) were positive for RSV, considering the three techniques. PCR was the most sensitive technique, providing positive findings in 35/316 (11.1%) of the samples, followed by direct immunofluorescence (25/316, 7.9%) and viral isolation (20/315, 6.3%) (p < 0.001). A sample was positive by immunofluorescence and negative by PCR, and 11 (31.4%) were positive only by RT-PCR. We conclude that RT-PCR is more sensitive than IF and viral isolation to detect RSV in nasopharyngeal aspirate specimens in newborn and infants.
对316份来自2岁及以下患有急性呼吸道疾病婴儿的鼻咽抽吸物样本，采用三种不同技术检测呼吸道合胞病毒（RSV）：病毒分离、直接免疫荧光法和聚合酶链反应（PCR）。考虑到这三种技术，36份（11.4%）样本RSV呈阳性。PCR是最敏感的技术，在316份样本中的35份（11.1%）检测出阳性结果，其次是直接免疫荧光法（25/316，7.9%）和病毒分离法（20/315，6.3%）（p<0.001）。有一份样本免疫荧光法呈阳性而PCR呈阴性，11份（31.4%）仅通过逆转录PCR呈阳性。我们得出结论，在检测新生儿和婴儿鼻咽抽吸物样本中的RSV时，逆转录PCR比免疫荧光法和病毒分离法更敏感。
