al-Khateeb T, Stephens P, Shepherd J P, Thomas D W
Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Jordan University of Science and Technology, Irbid, Jordan.
J Periodontol. 1997 Nov;68(11):1063-9. doi: 10.1902/jop.1997.68.11.1063.
To overcome the difficulties of studying wounding and wound repopulation in monolayer systems, a 3-dimensional model of wound repopulation has been developed which allows the in vitro investigation of fibroblast migration in response to experimental wounding. This model was utilized to determine whether fibroblasts derived from sites which demonstrate preferential healing (child and oral mucosal fibroblasts) possessed an increased ability to repopulate experimental wounds when compared to adult dermal fibroblasts. Fibroblasts were established from specimens derived from healthy donors undergoing minor elective surgery. Standard wounds were created in fibroblast populated collagen lattices (FPCLs) which were then overlaid upon an extracellular wound matrix. Fibroblast repopulation of the wounds was studied over 12 days using light- and scanning electron microscopy and quantified using computerized image analysis. Wound repopulation by fibroblasts derived from child donors (n = 3) was significantly (P < 0.001) more rapid than their adult tissue-matched counterparts (n = 3). Wound repopulation by oral mucosal fibroblasts (n = 3) was significantly greater than that exhibited by age-matched dermal fibroblasts (n = 3; P < 0.05). These differences were not reflected in differences in DNA synthesis (P > 0.5) or cell number (P > 0.5) within similar attached FPCL systems. These findings further support the concept of a gradual transition from the fetal to adult phenotype in wound healing. The potential applications of the model are discussed.
为克服在单层系统中研究创伤及伤口再填充的困难，已建立了一种伤口再填充的三维模型，该模型可用于体外研究成纤维细胞对实验性创伤的迁移反应。利用该模型来确定源自显示出优先愈合部位的成纤维细胞（儿童和成人口腔黏膜成纤维细胞）与成人真皮成纤维细胞相比，是否具有更强的再填充实验性伤口的能力。成纤维细胞取自接受小型择期手术的健康供体的标本。在接种有成纤维细胞的胶原晶格（FPCLs）中制造标准伤口，然后将其覆盖在细胞外伤口基质上。使用光学显微镜和扫描电子显微镜在12天内研究伤口的成纤维细胞再填充情况，并使用计算机图像分析进行量化。来自儿童供体的成纤维细胞（n = 3）对伤口的再填充明显（P < 0.001）比与其年龄匹配的成人组织对照（n = 3）更快。口腔黏膜成纤维细胞（n = 3）对伤口的再填充明显大于年龄匹配的真皮成纤维细胞（n = 3；P < 0.05）。在类似的贴壁FPCL系统中，这些差异并未反映在DNA合成（P > 0.5）或细胞数量（P > 0.5）的差异上。这些发现进一步支持了伤口愈合过程中从胎儿表型到成人表型逐渐转变的概念。文中讨论了该模型的潜在应用。
