Kerangueven F, Noguchi T, Coulier F, Allione F, Wargniez V, Simony-Lafontaine J, Longy M, Jacquemier J, Sobol H, Eisinger F, Birnbaum D
Laboratoire de Biologie des Tumeurs and CR Inserm 4U003C, Institut Paoli-Calmettes, Marseille, France.
Cancer Res. 1997 Dec 15;57(24):5469-74.
Deletions of genomic regions involving tumor suppressor genes are thought to be important in the initiation and progression of breast cancer. We conducted a genome-wide search for deleted regions in a series of 75 human breast carcinomas by studying the allelic patterns of 184 microsatellite markers distributed over all chromosomes and looking for loss of heterozygosity (LOH). We identified 56 regions of consistent LOH. Strikingly, every tumor had a different set of deletions. To study this complexity, we applied a phylogenetic-like type of analysis. Each region was involved in a certain proportion of tumors, ranging from 20 to 62%; the most frequently involved regions were on chromosome arms 8p, 11q, 16q, and 17p. There was a correlation (P = 0.005) between the level of LOH and the size of the tumors. Tumors with a high level of LOH were also highly proliferative and had a high mitotic index.
涉及肿瘤抑制基因的基因组区域缺失被认为在乳腺癌的发生和发展中起着重要作用。我们通过研究分布在所有染色体上的184个微卫星标记的等位基因模式并寻找杂合性缺失（LOH），对一系列75例人类乳腺癌进行了全基因组范围的缺失区域搜索。我们确定了56个一致出现杂合性缺失的区域。令人惊讶的是，每个肿瘤都有一组不同的缺失区域。为了研究这种复杂性，我们应用了一种类似系统发育的分析方法。每个区域在一定比例的肿瘤中出现，比例范围从20%到62%；最常出现缺失的区域位于染色体臂8p、11q、16q和17p上。杂合性缺失水平与肿瘤大小之间存在相关性（P = 0.005）。杂合性缺失水平高的肿瘤增殖性也很高，且有高有丝分裂指数。
