MacKenzie S H, Roberts M P, Liu K H, Doré J J, Godkin J D
Department of Animal Science, The University of Tennessee, Knoxville 37901-1071, USA.
Biol Reprod. 1997 Dec;57(6):1445-50. doi: 10.1095/biolreprod57.6.1445.
Retinol-binding protein (RBP) mRNA was localized to luminal and glandular epithelial cells of bovine endometrium by in situ hybridization. Relative levels of endometrial RBP mRNA expression during the estrous cycle and early pregnancy were determined by quantitative slot blot analysis and contrasted with uterine luminal concentrations of RBP. Expression of mRNA was moderate at Day 1 after estrus, declined at Day 5, reached lowest levels by Day 10, rose significantly through Days 15-17, and peaked at Day 20. RBP mRNA expression in pregnant animals was similar to that in cyclic animals on Day 15, doubled between Days 17 and 20, remained constant through Day 22, and rose slightly thereafter. Luminal RBP concentrations of cyclic cows, as determined by ELISA, decreased from Day 1 through Day 10, rose dramatically on Day 15, then declined through Day 20. Concentrations of RBP in uterine flushes from pregnant animals were similar to those of cyclic cows on Day 15 but remained relatively constant through Day 17. It is concluded that 1) RBP synthesis occurs in the luminal and glandular epithelial cells, 2) RBP transcription and secretion are correlated with each other, and 3) ovarian steroids, possibly in conjunction with uterine concentrations of their receptors, modulate uterine RBP expression.
通过原位杂交技术，视黄醇结合蛋白（RBP）mRNA定位于牛子宫内膜的腔上皮细胞和腺上皮细胞。通过定量狭缝印迹分析确定发情周期和妊娠早期子宫内膜RBP mRNA表达的相对水平，并与子宫腔内RBP浓度进行对比。发情后第1天mRNA表达适中，第5天下降，第10天达到最低水平，第15 - 17天显著上升，并在第20天达到峰值。妊娠动物的RBP mRNA表达在第15天与周期动物相似，在第17 - 20天翻倍，直至第22天保持恒定，此后略有上升。通过酶联免疫吸附测定法（ELISA）测定，周期奶牛的子宫腔内RBP浓度从第1天至第10天下降，第15天急剧上升，然后至第20天下降。妊娠动物子宫冲洗液中RBP的浓度在第15天与周期奶牛相似，但直至第17天保持相对恒定。得出以下结论：1）RBP在腔上皮细胞和腺上皮细胞中合成；2）RBP转录和分泌相互关联；3）卵巢类固醇可能与其受体的子宫浓度共同调节子宫RBP表达。
