Baskaran S, Kookana R S, Naidu R
Cooperative Research Centre for Soil and Land Management, Australia.
J Chromatogr A. 1997 Nov 7;787(1-2):271-5. doi: 10.1016/s0021-9673(97)00652-3.
We describe an analytical technique for measuring residues of imidacloprid, a relatively new and highly active insecticide, in water and soil using high-performance liquid chromatography (HPLC). All analyses were performed on reversed-phase HPLC with UV detection at 270 nm using a mobile phase of acetonitrile-water (20:80, v/v). Fortified water samples were extracted with either solid-phase extraction (SPE) or liquid-liquid extraction methods. A detection limit of 0.5 microgram/l was achieved using the SPE method. The imidacloprid residues in soils were extracted with acetonitrile-water (80:20, v/v), and the extract was then evaporated using a rotary evaporator. The concentrated extract was redissolved in 1 ml of acetonitrile-water (20:80, v/v) prior to analysis by reversed-phase HPLC. A detection limit of 5 micrograms/kg was obtained by this method which is suitable for analysis of environmental samples. Accuracy and precision at 10 and 25 micrograms/kg soil samples were 85 +/- 6% and 82 +/- 4%, respectively.
我们描述了一种使用高效液相色谱法（HPLC）测定水和土壤中吡虫啉（一种相对较新且活性高的杀虫剂）残留量的分析技术。所有分析均在反相HPLC上进行，使用乙腈 - 水（20:80，v/v）作为流动相，在270 nm处进行紫外检测。加标水样采用固相萃取（SPE）或液 - 液萃取方法进行萃取。使用SPE方法实现了0.5微克/升的检测限。土壤中的吡虫啉残留用乙腈 - 水（80:20，v/v）萃取，然后用旋转蒸发仪蒸发萃取液。在通过反相HPLC分析之前，将浓缩的萃取液重新溶解在1毫升乙腈 - 水（20:80，v/v）中。通过该方法获得了5微克/千克的检测限，该方法适用于环境样品的分析。在10微克/千克和25微克/千克土壤样品中的准确度和精密度分别为85±6%和82±4%。
