Obana Hirotaka, Okihashi Msahiro, Akutsu Kazuhiko, Kitagawa Yoko, Hori Shinjiro
Osaka Prefectural Institute of Public Health, 1-3-69 Nakamichi, Higashinari, Osaka, 5370025 Japan.
J Agric Food Chem. 2002 Jul 31;50(16):4464-7. doi: 10.1021/jf025539q.
Determination of 3 neonicotinoid insecticides, nitenpyram, imidacloprid, and acetamiprid, was studied. Vegetables and fruits were extracted with acetonitrile. The crude extract was passed through a weak anion-exchange cartridge (PSA). The effluent was subjected to silica gel cartridge. Imidacloprid and acetamiprid were eluted with 10 mL of 4:6 (v/v) acetone/hexane, followed by nitenpyram with acetone (20 mL). Pesticides were determined by HPLC with a C-18 column and diode-array detection system. Imidacloprid and acetamiprid were recovered at about 90% at the spike levels with 0.2 and 2 mg/kg in cucumber, potato, tomato, eggplant, Japanese radish, and grape. Nitenpyram was recovered at 64-80%. Relative standard deviations were less than 10% throughout all the recovery tests. In the residue analysis, agriculturally incurred pesticides at 0.08-0.14 mg/kg were designated with UV spectra compared with respective reference standards.
对3种新烟碱类杀虫剂烯啶虫胺、吡虫啉和啶虫脒的测定进行了研究。蔬菜和水果用乙腈提取。粗提取物通过弱阴离子交换柱（PSA）。流出物再通过硅胶柱。吡虫啉和啶虫脒用10 mL 4:6（v/v）丙酮/己烷洗脱，随后烯啶虫胺用丙酮（20 mL）洗脱。采用配有C-18柱和二极管阵列检测系统的高效液相色谱法测定农药。在黄瓜、土豆、番茄、茄子、白萝卜和葡萄中，添加水平为0.2和2 mg/kg时，吡虫啉和啶虫脒的回收率约为90%。烯啶虫胺的回收率为64%-80%。在所有回收率测试中，相对标准偏差均小于10%。在残留分析中，与各自的参考标准相比，利用紫外光谱对农业生产中产生的浓度为0.08-0.14 mg/kg的农药进行了鉴定。
