Sprando R L, Collins T F, Black T N, Rorie J, Ames M J, O'Donnell M
Division of Toxicological Research, Center for Food Safety Applied Nutrition, Food and Drug Administration, Beltsville, MD 20708, USA.
Food Chem Toxicol. 1997 Sep;35(9):881-90. doi: 10.1016/s0278-6915(97)88938-7.
The potential of sodium fluoride (NaF) to affect spermatogenesis and endocrine function was assessed in P and F1 generation male rats. Male and female experimental rats received sodium fluoride in their drinking water at one of four concentrations (25, 100, 175, 250 ppm). P generation male and female rats were exposed to sodium fluoride in their drinking water for 10 wk and then males were mated to females within the same treatment groups. Reproductive tissues were collected from P generation male rats after approximately 14 wk of treatment. Pregnant females (P) were exposed to sodium fluoride via their drinking water through gestation and lactation. F1 generation weanling male rats remained within the same treatment groups as their parents. F1 generation male rats were exposed to sodium fluoride in their drinking water for 14 wk, at which time reproductive tissues were collected. Dose-related effects were not observed within the P and F1 treatment groups in testis weights, prostate/seminal vesicle weights, non-reproductive organ weights, testicular spermatid counts, sperm production per gram of testis per day, sperm production per gram of testis, LH, FSH or serum testosterone concentrations. Histological changes were not observed in testicular tissues from either the P or F1 generation. We conclude that prolonged exposure to sodium fluoride in drinking water at the doses administered in this study does not adversely affect spermatogenesis or endocrine function in the P and F1 generation male rats.
在P代和F1代雄性大鼠中评估了氟化钠（NaF）对精子发生和内分泌功能的影响。雄性和雌性实验大鼠饮用含有四种浓度（25、100、175、250 ppm）之一的氟化钠的水。P代雄性和雌性大鼠在饮用水中接触氟化钠10周，然后将同一处理组内的雄性与雌性交配。在处理约14周后从P代雄性大鼠收集生殖组织。怀孕的雌性大鼠（P）在整个妊娠期和哺乳期通过饮用水接触氟化钠。F1代断奶雄性大鼠与它们的父母保持在相同的处理组中。F1代雄性大鼠在饮用水中接触氟化钠14周，此时收集生殖组织。在P代和F1代处理组中，未观察到睾丸重量、前列腺/精囊重量、非生殖器官重量、睾丸精子细胞计数、每克睾丸每天的精子产量、每克睾丸的精子产量、促黄体生成素（LH）、促卵泡生成素（FSH）或血清睾酮浓度与剂量相关的影响。在P代或F1代的睾丸组织中未观察到组织学变化。我们得出结论，在本研究中给予的剂量下，长期饮用含氟化钠的水不会对P代和F1代雄性大鼠的精子发生或内分泌功能产生不利影响。
